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ActivationofcAMP-DependentSignalingPathwayInducesMouseOrganicAnionTransportingPolypeptide2Expression
...on.Materials.ForskolinwasobtainedfromCalbiochem(LaJolla,CA).Di-butyrylcAMP(dbcAMP),8-bromocAMP(8-Br-cAMP),anddimethylsulfoxidewerepurchasedfromSigma(St.Louis,MO).Animals.Male6-week-oldC57BL6micewerepurchasedfromCharlesRiverLaboratories(Wilmington,MA)andmaintainedona12-hlight/darkcycle.Afteracclimati...
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蛋白激酶A对精子顶体酶活性的影响
...0在最大实验浓度(100nM)的条件下,能完全抑制由弗司扣林或dbcAMP刺激的顶体反应,而25nmol/L和50nmol/L浓度的KT5720对弗司扣林引起的顶体反应有58%的抑制作用,对dbcAMP引起的顶体反应则分别有50%和67%的抑制作用。 综上所述,PKA是第二...
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双丁酰环磷腺苷
...物名称双丁酰环磷腺苷药物别名双丁酰环化腺苷酸,DBC,DBcAMP英文名称DibutyrylCyclicAdenoslnePhosphate说 明注射剂:20mg功用作用环磷腺苷的衍生物,作用和用途与之相同。亦可用于心肌炎、心源性休克和手术后网膜下出血用法用...
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EnhancedFunctionalGapJunctionNeoformationbyProteinKinaseAeCDependentandEpac-DependentSignalsDownstreamofcAMPinCardiacMyocytes
...nincardiacmyocytes.MaterialsandMethodsReagentsandcAMPAnalogsDibutyryl-cAMP(dbcAMP)waspurchasedfromSigma-Aldrich,Epac-specificactivator8-(4-chlorophenylthio)-2‘-O-methyladenosine-3‘,5‘-cyclicmonophosphate(8CPT)fromCalbiochem;andPKA-specificactivatorN6-benzoyladenosine-3‘,5‘-cyclicmonophosph...
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NorepinephrineInducesLipolysisin1/2/3-AdrenoceptorKnockoutMice
...,Switzerland).AdenosinedeaminasewasfromRoche(Meylan,France).Dibutyryl-cAMP(dbcAMP)wasfromRoche.(-)-BupranololwasfromSchwarzPharma(Monheim,Germany).CL316243[GenBank]wasfromtheMedicalResearchDivision,LederleLaboratories,AmericanCyanamidCompany(PearlRiver,NY).PrazosinwasfromPfizer,Inc.(LaJolla,CA).(±)...
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AVP-inducedVIT32geneexpressionincollectingductcellsoccursviatrans-activationofaCREinthe5‘-flankingregionoftheVIT32gene
...lcollagenwerepurchasedfromSigma(St.Louis,MO).Forksolin,IBMX,dibutyryl-cAMP(dbcAMP),andH89werefromEMDBiosciences(SanDiego,CA).ActinomycinDwasobtainedfromRocheBiochemicals(Indianapolis,IN),polydI-dCfromPharmacia(Piscataway,NJ),andRU-38486wasagenerousgiftfromRousselUclaf(Romainville,France).Culturemate...
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SuppressorofCytokineSignaling-AntagonizescAMPEffectsonProliferationandApoptosisandIsExpressedinHumanProstateCancer
...AssayCells(LNCaP-IL-6+,PC3)wereseededatadensityof1x104/wellandincubatedwithdbcAMP(1to5mmol/L;Sigma,Vienna,Austria)for48and72hoursintriplicatesin96-wellplates,and50µlofthymidine(1µCi/well)wereadded.Aftera16-hourincubation,cellswerefrozenatC18??C.Afterthawing,DNAwasharvestedon96-wellfilter...
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骨髓间充质干细胞治疗脑梗死的研究进展
...经细胞培养基中加入多种脑源性神经营养因子hEGF、bFGF、dbcAMP、IBMX、BDNF和bFGF8可以直接诱导BMSCs分化成神经样细胞。2006年Mareschi等[15]比较了几种条件培养基的神经分化效果:最低条件培养基/β巯基乙醇,最低条件培养...
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骨髓基质细胞神经分化潜能与脑缺血性疾病
...丁基-1-甲基黄嘌呤(IMBX)和cAMP类似物双丁酰环磷腺苷(dbcAMP)。2天后,MSCs开始出现形态学改变,胞体圆缩,突起延长变细。诱导6天,发现近25%的MSCs分化为典型的神经细胞形态;并且提高了NSE和房肽素的表达,采用Westernblot检...
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DesensitizationoftheDopamineD1andD2ReceptorHetero-OligomerMediatedCalciumSignalbyAgonistOccupancyofEitherReceptor
....Forproteinkinaseactivatorstudies,cellswerepretreatedwith3mMdibutyryl-cAMP(dbcAMP)(Calbiochem)for15minorwith1µMphorbol12-myristate13-acetate(PMA;Calbiochem)for30minbeforecalciummeasurement.SKF81297,dopamine,quinpirole,SCH23390,raclopride,ADPβS,EGTA,SQ22536,andpertussistoxinwerepurchasedfr...
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骨髓干细胞移植治疗心肌梗死对交感神经重构的影响
...胞分化,即在加入B27、双丁酰环腺苷酸(dibutyrylcyclicAMP,dbcAMP)、异丁基甲基黄嘌呤(isobutylmethylxanthine,IBMX)、FGF-8、脑源性神经营养因子(brainderivedneurotrophicfactor,BDNF)、人表皮生长因子(humanepidermalgrowthfactor,hEGF)和碱性FGF(basicFGF,bF...
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FenofibricAcid,anActiveFormofFenofibrate,IncreasesApolipoproteinA-I–MediatedHigh-DensityLipoproteinBiogenesisbyEnhancingTranscriptionofATP-Bi
...nd0.2%BSA.Duringthelast24hoursofthedrugtreatment,300μmol/LofdibutyrylcAMP(dbcAMP;Wako)andapoA-I(10μg/mL)wereaddedtothemedium.25THP-1cellsandBALB/3T3cellswerealsotreatedwiththePPARactivatorsandapoA-Iin0.2%BSA-RPMI1640mediumand0.1%BSA-MEM,respectively.Cholesterolandcholine-phospholipidreleasedintoth...
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VerapamilIncreasestheApolipoprotein-MediatedReleaseofCellularCholesterolbyInductionofABCA1ExpressionViaLiverXReceptor-IndependentMechanism
...Thecellsweresubculturedin6-wellplatesandtreatedwith300μmol/LdibutyrylcAMP(dBcAMP)inDMEM/F-12(1:1)containing0.1%bovineserumalbuminfor18hours,andforadditionaltimeinthesamemediuminthepresenceorabsenceofverapamil(WakoPureChemicals,Tokyo,Japan),andind-verapamilandl-verapamil(kindgiftsfromKnollAG,Ludwigs...
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ThromboxaneA2PromotesInterleukin-6BiosynthesisMediatedbyanActivationofCyclicAMP-ResponseElement-BindingProteinin1321N1HumanAstrocytomaCells
...r,MI).ProstaglandinE2,Ro20-1724,isoproterenol,carbachol,dibutyrylcyclicAMP(dbcAMP),H89,wortmannin,andKN62werepurchasedfromSigma-Aldrich(St.Louis,MO).IL-6enzyme-linkedimmunosorbentassay(ELISA)kitwasfromAmershamBiosciencesInc.(Piscataway,NJ).RNAextractionkitwasfromNipponGeneCo.,Ltd.(Toyama,Japan),andr...