夏天无生物碱的高速逆流色谱分离纯
...ethylacetatemethanolwater(5∶5∶2∶8,V/V),inwhich5mmol/Ltriethylamineintheorganicstationaryphaseand5mmol/Lhydrochloricacidintheaqueousmobilephase.From3.0gofcrudeextract,amixtureandthreecompounds,with375mgprotopine(1),362mghumosineA(2)and246mgbicuculline(3)wereobtainedwithpuritiesof97.5%,95....
药品天地;专业药学;实验技术;色谱技术;色谱分析实例TheBreastCancerResistanceProtein(Bcrp1/Abcg2)LimitsFetalDistributionofGlyburideinthePregnantMouse:AnObstetric-FetalPharmacologyResearchUnit
...iabeticdrugroutinelyusedtotreatgestationaldiabetes.Inthisstudy,wefirstdeterminedwhetherGLBisaBCRP/Bcrp1substrate.Theintracellular[3H]GLBconcentrationsinMadin-Darbycaninekidney(MDCK)/BCRPcellsweresignificantlylowerthanthoseinMDCK/vectorcells.Theadditionof10µMfumitremorginC,aspecificBCRPinhibito...
医源资料库;在线期刊;分子药理学杂志;2008年第70卷第3期高效液相色谱法测定葛根、红花和山楂混合提取物中葛根素、羟基红花黄色素A的含量
...酸水溶液∶甲醇∶乙腈(81∶16∶3)为流动相,流速1.2ml/min,柱温25℃,检测波长分别为250,403nm,测葛根素和羟基红花黄色素A。结果葛根素和羟基红花黄色素A线性范围分别为0.032406~0.16208mg/ml和0.0294~0.147mg/ml;平均加样回收率分...
药品天地;专业药学;实验技术;色谱技术;色谱论文高效液相色谱法同时测定血清中头孢哌酮和舒巴坦的含量
...化铵—乙腈(825∶175,V/V),磷酸调整pH至5.0,流速为1.0mL/min,在紫外检测器230nm进行检测。本法的头孢哌酮和舒巴坦的最低检出浓度分别为10mg/L和5mg/L,至少在10~1000mg/L范围内呈线性,头孢哌酮和舒巴坦的绝对回收率分别是99.4%...
药品天地;专业药学;实验技术;色谱技术;色谱论文山羊骨髓间充质干细胞的分离培养及鉴定
...度为1.07g/mL的Percoll(Amresco)分离液的离心管中,以3000r/min离心20min,吸取中间乳白色云雾状有核细胞层,漂洗后加入DMEM/F12培养基(Hyclone)和体积比10%的胎牛血清(Hyclone),制成单细胞悬液,以2×106/cm2的细胞密度接种培...
医源资料库;在线期刊;局解手术学杂志;2008年第17卷第6期异丙酚复合氯胺酮在小儿眼科手术气管插管全静脉麻醉中的应用
...组)和吸入组(H组),每组各30例。1.2麻醉方法患儿术前30min阿托品(Atropine)0.01~0.02mg/kg肌注,异丙酚(propofol)2.5mg/kg,氯胺酮(Ketamine)1~2mg/kg,维库溴胺(Vecuronium)0.1mg/kg静脉诱导,90s后气管内插管,接Drager-Julian麻醉机行机械...
医源资料库;在线期刊;中华医药杂志;2006年第6卷第8期腰-硬联合麻醉在下肢骨折手术中的应用
...EA),Ⅱ组为连续硬膜外麻醉组(EA),两组均于麻醉前30min肌注苯巴比妥钠0.1g,阿托品0.5mg。CSEA组局麻药为0.75%布比卡因0.2mg/kg,两组硬膜外局麻药均为1.33%~2%利多卡因。分别记录两组病人的麻醉显效时间、局麻药用量,麻醉效...
医源资料库;在线期刊;中华现代外科学杂志;2007年第4卷第5期MidazolamMetabolisminCytochromeP4503AKnockoutMiceCanBeAttributedtoUp-RegulatedCYP2CEnzymes
...rgrouphasgeneratedcytochromeP4503Aknockoutmicetostudythisdrug-handlingsysteminvivo.Inthepresentstudy,wehavecharacterizedtheCyp3aknockoutmicebystudyingthemetabolismofmidazolam,oneofthemostwidelyusedprobestoassessCYP3Aactivity.WeexpectedthatthemidazolammetabolismwouldbeseverelyreducedintheabsenceofCYP...
医源资料库;在线期刊;分子药理学杂志;2008年第70卷第3期E-Ring8-IsoprostanesAreAgonistsatEP2-andEP4-ProstanoidReceptorsonHumanAirwaySmoothMuscleCellsandRegulatetheReleaseofColony-StimulatingFac
...s,respectively.Todate,themajorityofinvestigationshave,almostexclusively,examinedtheeffectofisoprostanesonsmoothmuscletone.Herein,wedescribeananti-andproinflammatoryeffectofE-ring8-isoprostanesonthesyntheticcapacityofHASMcellsandhaveidentifiedthereceptorsandsignaltransductionpathwaysinvolved.Inpartic...
医源资料库;在线期刊;分子药理学杂志;2005年第67卷第2期梯度洗脱HPLC同时测定鼻腔洗剂Ⅰ号中甲硝唑和氯霉素含量
...B两种,A为8%乙腈(V/V)、B为乙腈。梯度洗脱程序:0—10min,A100%—37%;11—16min,A37%—100%;流速1.6ml/min,检测波长286nm。在此色谱条件下,测得甲硝唑和氯霉素对照品混合溶液、鼻腔洗剂Ⅰ号样品和不含甲硝唑和氯霉素的阴性样...
药品天地;专业药学;实验技术;色谱技术;色谱论文