TheThree-SubstitutedIndolinoneCyclin-DependentKinase2Inhibitor3-[1-(3H-Imidazol-4-yl)-meth-(Z)-ylidene]-5-methoxy-1,3-dihydro-indol-2-one(SU9516)Kill
...µMSUfor0,1,2,4,and6h.Aftertreatment,totalcellularextractsandcytosolicS100fractionswerepreparedandsubjectedtoWesternblotassayusingantibodiesagainstPARP,cleaved-caspase-3,caspase-8,andcytochromecasdescribedunderMaterialsandMethods.Eachlanewasloadedwith30µgofprotein.Blotsweresubsequentlystr...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第8期阿柔比星缓释制剂的制备工艺研究
...种移植性动物肿瘤如L310、P388、Ehlrich腹水癌、Lewis肺癌、S100肉瘤、B16黑色素瘤和CDF8及C3H乳癌等均有较强的抗瘤活性,对卵巢癌和胃癌也有疗效。阿柔比星能抑制癌细胞的生物大分子合成,特别是对RNA合成的抑制作用很强。阿柔比...
医源资料库;在线期刊;中国热带医学杂志;2008年第8卷第7期PlitidepsinCellularBindingandRac1/JNKPathwayActivationDependonMembraneCholesterolContent
...ltracentrifugetosedimentparticulatematerial.Thesupernatant(solublefraction,S100)wasremoved,andthepellet(particulatefraction,P100)wasresuspendedinhypotoniclysisbuffer.Proteinconcentrationsinthefractionsweredeterminedusingacolorimetricassay(Bio-RadLaboratories,Hercules,CA).[14C]Plitidepsin.14C-labeled...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第11期ALOX5APGeneandthePDE4DGeneinaCentralEuropeanPopulationofStrokePatients
...sthaninfemales,withSG13S114(oddsratio,1.24;95%CI,1.04to1.55;P=0.017)andSG13S100(oddsratio,1.26;95%CI1.03to1.54;P=0.024)showingthestrongestassociations.NosignificantassociationsweredetectedwithsinglemarkersandhaplotypesinPDE4D.Thefrequenciesofsingle-markerallelesandhaplotypesdifferedlargelyfromthosei...
医源资料库;在线期刊;中风学杂志;2005年第36卷第4期树突状细胞与脑胶质瘤免疫治疗的研究进展
...合素、B7、CD40、IL-12等;(4)DC的限制性标志为CD83、P55、S100b等。骨髓及血液中的前体DC随血流分布于皮肤、胃肠道、心、肝、肺等非淋巴器官发育为未成熟DC,主要参与抗原的摄取、加工和处理,高表达CD86和HLA-DR等,这时期抗...
资料库;在线期刊;中华现代外科学杂志;2005年第2卷第14期ThePsoriaticTranscriptomeCloselyResemblesThatInducedbyInterleukin-inCulturedKeratinocytes
...prisingmoleculesfoundontheepidermaldifferentiationcomplexonchromosome1q(eg,S100A7,SPRR1B),moleculeswithantimicrobialactivity(eg,DEFB4,LCN2),hyperproliferation-associatedmolecules(eg,KRT6A,KRT16),andmoleculesinvolvedintheregulationofproteolysis(eg,SERPINB3,PI3).Inaddition,numerouscytokinesorcytokine-...
医源资料库;在线期刊;美国病理学杂志;2007年第169卷第7期EffectofcombinationtherapywithenalaprilandtheTGF-antagonist1D11inunilateralureteralobstruction
...ingimmunohistochemistry,aspreviouslydescribed(8).TheantibodyutilizedwasDAKOS100-A4(1:100dilution).TheS100-A4antigenisalsoknownasFSP-1(1,32).AntigenwasretrievedbyincubatingcellswithproteinaseKfor20mininanoven.Theremainingimmunoperoxidaseprotocolwascarriedoutasroutinelydoneinourlaboratory.StainingforS...
医源资料库;在线期刊;美国生理学杂志;2007年第290卷第4期InterdependentSerotoninTransporterandReceptorPathwaysRegulateS100A4/Mts1,aGeneAssociatedWithPulmonaryVascularDisease
...orth),UniversityofSheffield,UnitedKingdom.AbstractHeightenedexpressionoftheS100calciumeCbindingprotein,S100A4/Mts1,isobservedinpulmonaryvasculardisease.Lossofserotonin(5-hydroxytryptamine[5-HT])receptorsoroftheserotonintransporter(SERT)attenuatespulmonaryhypertensioninanimals,andpolymorphismscausing...
医源资料库;在线期刊;循环研究杂志;2005年第95卷第8期Retinoid-InducedApoptosisinHL-60CellsIsAssociatedwithNucleolinDown-RegulationandDestabilizationofBcl-2mRNA
...rotein.Bindingofabcl-2mRNAinstabilityelement(AU-richelement-1)tonucleolininS100extractsfromATRA-treatedcellswasdecreasedto15%ofcontrolwithin72h.Thedecayof5‘capped,polyadenylatedbcl-2mRNAtranscriptscontainingARE-1wasmorerapidinS100extractsfromATRA-treatedcellscomparedwithuntreatedcells.However,when...
医源资料库;在线期刊;分子药理学杂志;2005年第67卷第1期Injury-inducedinnateimmuneresponseinhumanskinmediatedbytransactivationoftheepidermalgrowthfactorreceptor
...vity.J.Biol.Chem.270:1015–1019.Gl?serR.,etal.2005.Antimicrobialpsoriasin(S100A7)protectshumanskinfromEscherichiacoliinfection.Nat.Immunol.6:57–64.BevinsC.L.2005.Animportantclue:fingerprintspointtopsoriasinindefenseagainstE-coli.Nat.Immunol.6:12–13.TokumaruS.,etal.2000.Ectodomainsheddingofepide...
医源资料库;在线期刊;临床研究杂志;2006年第116卷第7期