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Cyclicalmodulationofsphingosine-1-phosphatereceptor1surfaceexpressionduringlymphocyterecirculationandrelationshiptolymphoidorgantransit
...oviralinfectionwasperformedbyincubatingtotalsplenocytesat107permlfor20–24hon24-wellplatesprecoatedfor2–3hat37°Cwith3μg/mlanti-CD3and0.5μg/mlanti-CD28(BDBiosciences).ActivatedTcellswerespininfectedat2,450RPMinaBeckmanCoulterAllegra6Rcentrifugefor2hat25°Casdescribedpreviously(42),andthencultur...
医源资料库；在线期刊；实验医学杂志；2005年第201卷第2期 -
TheThiazolidinedionePioglitazoneAltersMitochondrialFunctioninHumanNeuron-LikeCells
...reusedtotreatorarebeingconsideredforthetreatmentofdisordersthatfeaturemitochondrialimpairment.Theirmitochondrialeffects,however,havenotbeencomprehensivelystudiedunderlong-termexposureconditions.Weusedthehumanneuron-likeNT2celllinetodirectlyassessthelong-termeffectsofathiazolidinedionedrug,pioglitazo...
医源资料库；在线期刊；分子药理学杂志；2007年第69卷第6期 -
Microarray-BasedDetectionof90AntibioticResistanceGenesofGram-PositiveBacteria
...eotideschosenfromconsensussequencesofgenefamilies,exceptforninegenesforwhichonlyonespecificoligonucleotidecouldbedeveloped.Atotalof137oligonucleotides(26to33nucleotidesinlengthwithsimilarphysicochemicalparameters)werespottedontothemicroarray.Themicroarrays(ArrayTubes)werehybridizedwith36strainscarry...
医源资料库；在线期刊；微生物临床杂志；2005年第43卷第5期 -
-Tocopheroldisappearanceisfasterincigarettesmokersandisinverselyrelatedtotheirascorbicacidstatus
...lectiontoalleviatethetransientoxidativestresseffects.Urinewascollectedfor24hon3occasions:beforesupplementation(d–6)andondays0and17.Afterthetotalurinevolumewasdetermined,aliquotswerecollectedandstoredat–40°Cuntilanalysis.BloodsamplehandlingBloodtubeswithadditiveswerekeptonicefor30minbe...
医源资料库；在线期刊；美国临床营养学杂志；2005年81卷第1期 -
GenomicApproachtoIdentificationofMycobacteriumbovisDiagnosticAntigensinCattle
...reachantigenatafinalconcentrationof4µg/ml.Duplicatecontrolwellstowhichonlyphosphate-bufferedsalinewasaddedwerealsoestablished.Becausethecoefficientofvariationbetweenduplicatewellshasbeenfoundtobelessthan5%(31),culturesupernatantswerepooledbeforetestingintheIFN-ELISA.Theopticaldensityforphospha...
医源资料库；在线期刊；微生物临床杂志；2003年第41卷第8期 -
UncouplingofvasopressinsignalingincollectingductsfromratswithCBL-inducedlivercirrhosis
...ringunstressedconditions,theratswereadaptedtorestrainingcagesbytrainingfor2hon2consecutivedays.Bloodsamples(1ml)weretransferredtoTransylol/EDTAtubes;plasmawasisolatedbycentrifugationat4,000gandstoredat-20°Cuntilanalysis.AVPwasextractedfromplasmaonC-18SEP-PakcartridgesandmeasuredbyRIAaspreviously...
医源资料库；在线期刊；美国生理学杂志；2004年第287卷第10期 -
CholesterylEsterTransferProtein(CETP)InhibitionBeyondRaisingHigh-DensityLipoproteinCholesterolLevels
...scusstheeffectsofCETPinhibitiononfecalsterolexcretion.Finally,webrieflytouchontheeffectsofCETPinhibitionontheanti-inflammatoryandantioxidativepropertiesofHDL.CETPInhibitionandRCTRCTisgenerallyinvokedtoprovidetherationalefortheantiatherogenicpropertiesofHDL.Itconcernstheremovalofexcesscholesterolfrom...
医源资料库；在线期刊；动脉硬化血栓血管生物学杂志；2006年第26卷第4期 -
Afeasibilitystudyquantifyinginvivohuman-tocopherolmetabolism
...singisshowninFigure2.Chylomicrawereseparated(fromVLDL)fromblooddrawnat4and6honly.Forpurposesofpresentation,VLDLwasstackedatopthechylomicra(sothattheheightaboveandbelowthewhitestripe=VLDL:chylomicra).Ingeneral,chylomicraandVLDLlabelingwith14Cweresimilarforbothisomers.TheVLDL:chylomicratendedtobesmall...
医源资料库；在线期刊；美国临床营养学杂志；2006年84卷第6期 -
Oxidativestress,renalinfiltrationofimmunecells,andsalt-sensitivehypertension:allforoneandoneforall
...fusedtoheatshockcognateprotein70.IntImmun13:1233-1242,2001.VaziriND,DicusM,HoN,andSindhuRK.Oxidativestress,anddysregulationofsuperoxidedismutase,NAD(P)Hoxidaseandxanthineoxidaseinchronicrenalinsufficiency.KidneyInt63:179-185,2003.VaziriND,DingY,andNiZ.Compensatoryup-regulationofnitricoxidesynthaseis...
医源资料库；在线期刊；美国生理学杂志；2004年第287卷第4期 -
AutoantibodiestoVimentinCauseAcceleratedRejectionofCardiacAllografts
...ionsofsurfacestructuresonapoptotickeratinocytes.JExpMed1994,179:1317-1330MahoneyJA,RosenA:Apoptosisandautoimmunity.CurrOpinImmunol2005,17:583-588OhSI,KimIW,JungHC,SeoJW,ChaeIH,KimHS,OhBH:CorrelationofFasandFasligandexpressionwithrejectionstatusoftransplantedheartinhuman.Transplantation2001,71:906-90...
医源资料库；在线期刊；美国病理学杂志；2007年第169卷第4期