RapidIdentificationofStaphylococcalStrainsfromPositive-TestingBloodCultureBottlesbyInternalTranscribedSpacerPCRFollowedbyMicrochipGelElectr
...eciesisolatedfrombloodcultureswereS.epidermidis(76%),S.capitis(11%),andS.hominis(8%).Overall,all280bloodculturebottlesshowntocontainasingleStaphylococcusspeciesbyroutinephenotypicmethodswerecorrectlyidentifiedbythePCR-MGEmethodatthespecieslevel,whereastheorganismfailedtobeidentifiedin8culturebottles...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第3期RapidGenotypingofHepatitisCVirusbyPrimer-SpecificExtensionAnalysis
...RE33R2,CanadaABSTRACTQuickandaccurategenotypingofhepatitisCvirus(HCV)isbecomingincreasinglyimportantforclinicalmanagementofchronicinfectionandasanepidemiologicalmarker.Furthermore,theincidenceofHCVinfectionwithmixedgenotypeshasclinicalsignificancethatisnotaddressedbymostgenotypingmethods.Wehavedevel...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第10期PhylogeneticCharacterizationofCanineDistemperVirusesDetectedinNaturallyInfectedDogsinNorthAmerica
...racterizetheCDVstrainsdetectedinthefourcases,completegenesequencesweredeterminedforthehemagglutinin(H)andfusion(F)proteingenes,whilepartialgenesequencingwasperformedforthephosphoproteingene.Atotalof4,508basesweresequencedfortheCDVstrainsdetectedfromeachofthefourcases.Twocaseswerefoundtohaveidentical...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第10期地高辛配基随机标记DNA探针
...成分和体积要相应增加。 (1)DNA探针热变性,煮沸10min,迅速冷却于冰/乙醇中5min以上,待用。 (2)取Eppendorf管(1.5ml)置于冰上,加下列及试剂: 新鲜变性的DNA 1-3μg 六聚核苷酸混合物 ...
医药经济;生物技术;实验技术;实验室试剂仪器SuperoxidescavengingattenuatesrenalresponsestoANGIIduringnitricoxidesynthaseinhibitioninanesthetizeddogs
...ide(O2)andnitricoxide(NO)interactioninmediatingtherenalactionsofANGII,weexaminedtherenalresponsestointra-arterialinfusionofANGII(0.5ng・kg1・min1)beforeandduringadministrationofasuperoxidedismutasemimetic,tempol(0.5mg・kg1・min1),inthepresenceorabsenceofNOsynthaseinhibitor,nitro-L-arginine(NLA;5...
医源资料库;在线期刊;美国生理学杂志;2005年第288卷第2期ProspectiveEstimationofRecombinationSignalEfficiencyandIdentificationofFunctionalCrypticSignalsintheGenomebyStatisticalModeling
...rrelatedwithsequenceconservation:maximumIis1andindicatesstrictconservation;minimumIis0andindicatesthatnonucleotideismorefrequentthananyother.StatisticalModelsofRSStructure.WedevelopedstatisticalmodelsofRScorrelationstructurefor12-RSand23-RS(19).Briefly,eachmodelcomputesascoreforanysequenceofappropri...
医源资料库;在线期刊;实验医学杂志;2003年第197卷第2期ProspectiveEstimationofRecombinationSignalEfficiencyandIdentificationofFunctionalCrypticSignalsintheGenomebyStatisticalModeling
...rrelatedwithsequenceconservation:maximumIis1andindicatesstrictconservation;minimumIis0andindicatesthatnonucleotideismorefrequentthananyother.StatisticalModelsofRSStructure.WedevelopedstatisticalmodelsofRScorrelationstructurefor12-RSand23-RS(19).Briefly,eachmodelcomputesascoreforanysequenceofappropri...
医源资料库;在线期刊;美国病理学杂志;2003年第162卷第2期AMethodforAssayingDeubiquitinatingEnzymes
...clonedandexpressedinthecells.INTRODUCTIONUbiquitin(Ub)isahighlyconserved76aminoacidproteinfoundinalleukaryoticcells(1,2).Ubiscovalentlyligatedtoavarietyofintracellularproteinsthroughanisopeptidelinkage.UbsbythemselvesorthathavealreadybeenconjugatedtoproteinsmayalsobeligatedtoadditionalUbmoleculestof...
药品天地;专业药学;实验技术;生化实验;酶学实验刺五加胶囊增强小鼠免疫功能的研究
...yofthemiceanti-body-producingcellandthesheeperythrocyteantibodylevelsinserumincreased;theabilityofmononuclearmacrophageeliminatingcarbonandmononuclearceliacmacrophagephagocyticactivitywereraised,andthedelayedallergywasaccelerated.ConclusionItissuggestedthatciwujiacapsulecouldenhanceimmunefunction....
医源资料库;在线期刊;中华中西医杂志;2005年第6卷第15期1000例末节断指再植的观察与护理
...合剂的应用:氯丙嗪注射液50mg+异丙嗪注射液50mg。(3)20~30min,每次有效地按摩挤压,可促进静脉的回流,避免了代谢物的堆积,减少了代谢产物对组织的损害,减轻了动脉的搏出压力。(4)及时的滴血疗法,对于Ⅰ级、Ⅱ级病人,...
医源资料库;在线期刊;中华现代护理学杂志;2011年第8卷第13期