InfectiveEndocarditisCausedbyGranulicatellaelegansOriginatingintheOralCavity
....elegansCCUG26024(Table1).Amongthecharacteristicsexamined,ureaseproduction(25),acidificationofraffinoseandsucrose(7,22),andhydrolysisofhippurate(7)havebeenreportedtobestraindependent.StrainIMU02p18,aswellasstrainIMU02b01,werenegativeforurease,andthetwoisolatesfermentedsucrosebutnotraffinose.Inadditi...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第3期UseofrMPB70ProteinandESAT-6PeptideasAntigensforComparisonoftheEnzyme-LinkedImmunosorbent,Immunochromatographic,andLatexBeadAgglutinationAs
...econtainingtheimmunodominantportionofESAT-6(earlysecretoryantigenictarget)(25),aproteinsecretedduringtheearlyoractivephaseofmycobacterialinfection.TheantigenisnotpresentinM.aviumsubsp.paratuberculosis,andthegeneencodingithasbeendeletedinBCG(9,52,57,58).SincetheLBAAneedsonlyserum,latexbeadsconjugated...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第9期IdentificationofSwineHepatitisEVirus(HEV)andPrevalenceofAnti-HEVAntibodiesinSwineandHumanPopulationsinKorea
...ificprevalence.TheantibodyprevalenceratestotheswineHEVwere6.0,10.0,36.0,and25.0%,in2-,3-,4-,and5-to-7-month-oldpigs,respectively.Inaddition,theseroprevalenceinsowstotheswineHEVwas8.8%.Ontheotherhand,18%ofblooddonorsinKoreawerefoundtobepositiveforanti-HEVantibodies.Overall,thisstudyindicatesthatsubcl...
医源资料库;在线期刊;微生物临床杂志;2003年第41卷第8期DetectionandIdentificationofEhrlichiaspp.inTicksCollectedinTunisiaandMorocco
...eof50μlcontaining0.2μMeachprimer,200μMeachdeoxynucleosidetriphosphate,1.25UofTaqpolymerase(Q.Biogene),and1xTaqbuffer(1.5mMMgCl2).TheamplificationreactionwascarriedoutinaTouchDownthermalcycler(Hybaid)underthefollowingconditions:initialdenaturationat93°Cfor1min;35cycles,eachincludingdenaturationat...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第3期GenomicDeletionsClassifytheBeijing/WStrainsasaDistinctGeneticLineageofMycobacteriumtuberculosis
...,Stanford,California94305TuberculosisCenter,PublicHealthResearchInstitute,225WarrenSt.,Newark,NewJersey07103-3535MycobacteriaReferenceDepartment,DiagnosticLaboratoryforInfectiousDiseasesandPerinatalScreening,NationalInstituteofPublicHealthandtheEnvironment,Bilthoven,TheNetherlandsABSTRACTBeijing/Wst...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第7期Kidneystonedisease
...dexcretionratesamongidiopathicCaOxSFsclusteratthehighendofthisdistribution(25).Loweringurinarycalciumlevelspreventsstonerecurrence(12).Conventionalupperlimitsof250mg/d(forwomen)and300mg/d(formen)arenearthe95thpercentilesforourpublisheddatasets(26)andarecommonlyusedasdiagnosticcutpoints.WebelieveSFsi...
医源资料库;在线期刊;临床研究杂志;2005年第115卷第10期RapidandSensitiveDetectionofSevereAcuteRespiratorySyndromeCoronavirusbyRollingCircleAmplification
...enomicDNAwasdenaturedat95°Cfor5min,andaligationstepthenperformedat45°Cfor25min.Afterligationandcircularizationoftheprobe,asecondstepwasperformedtoamplifythecircularprobebyRCAbyusingDNApolymerasewithstrand-displacingactivity.TwoDNApolymeraseswerechosentoperformtheRCA:Vent(exo-)DNApolymeraseandBstDN...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第5期IdentificationofNovelHumanHepatitisEVirus(HEV)IsolatesandDeterminationoftheSeroprevalenceofHEVinKorea
...orea(8).Also,acaseofHEVinfectionwithclinicalsymptomshasbeenreportedinKorea(25).TheaimofthisstudywastoidentifyhumanHEVstrainsinhumanseraandtocharacterizetheprevalenceofanti-HEVantibodiesinhumans.Inthisstudy,15humanHEVstrainswereisolatedandphylogeneticallyanalyzed,andtheserologicalprevalenceofanti-HEV...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第7期ComparativeSequencingoftheSerine-AspartateRepeat-EncodingRegionoftheClumpingFactorBGene(clfB)forResolutionwithinClonalGroupsofStaphylococc
...tS.aureusstrainassociatedwithhealthcare-relatedinfectionsintheUnitedStates(25)andalsothestrainthatrecentlyhasacquiredresistancetovancomycin(55),areendemic.Inordertoovercomethisconstraint,ahighlyinformativelocuscapableofdiscriminatingwithinclonalgroupsisnecessary.ForspeciessuchasBacillusanthracis,mar...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第8期UtilityofMycobacterialInterspersedRepetitiveUnitTypingforDifferentiatingMultidrug-ResistantMycobacteriumtuberculosisIsolatesoftheBeijingFamily
...nsoftheBeijinggenotypefamily,102multidrug-resistant(MDR)clinicalisolatesand253randomlyselectednon-MDRisolatescollectedfrom2000to2003inHongKongweresubjectedto12-locusMIRUtyping,spoligotyping,andIS6110restrictionfragmentlengthpolymorphism(RFLP)typing.Spoligotypingshowedthat243(68.5%)of355isolatesbelon...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第1期