Integrationofflow-dependentendothelialphenotypesbyKruppel-likefactor2
...ndHL-60cells,allthecellswerelysedwith200μllysisbuffer(0.1%sodiumhydroxide/0.01%SDS)andquantifiedforfluorescence.Fortheoxidativestressassay,HUVECswerepreconditionedunderstatic(noflow)oratheroprotectiveflowfor24hours,thentreatedfor4hourswith100μMt-butylhydrogenperoxide(Calbiochem)inHUVECculturemediu...
医源资料库;在线期刊;临床研究杂志;2006年第116卷第1期AframeshiftpolymorphisminP2X5elicitsanallogeneiccytotoxicTlymphocyteresponseassociatedwithremissionofchronicmyeloidleukemia
...erusingthe9-merpeptideTPNQRQNVC.BeforeDLI,tetramer+cellscouldnotbedetected(0.01%)intheperipheralbloodofCMLpatientUPN389(datanotshown),whereasLRH-1–specificCD8+TcellswereclearlydetectableafterDLI-2(Figure4).ThePBMCsamplethatwasusedforisolationofCTLRP1contained0.27%oftetramer+cellsamongCD8+Tcells.Th...
医源资料库;在线期刊;临床研究杂志;2005年第115卷第12期第二节 染色方法
...抗体的ICC染色。 (14)呈色:HRP标记抗体的呈色液为0.01%~0.1%H2O20.01%~0.05%DAB0.05~0.1mol/LTris–HCl(pH7.4)。切片经PBS或Tris-HCl液漂洗后,置上述呈色液内10~15min(室温、暗处),亦可镜下控制显色速度。终产物为棕褐色沉淀。用...
医源资料库;医源图书馆;教材类;实用免疫细胞与核酸PositiveSelectionofAnti–Thy-1AutoreactiveB-1CellsandNaturalSerumAutoantibodyProductionIndependentfromBoneMarrowBCellDevelopment
...endedat5x106/mlandloadedwith8µMIndo-1a.m.()togetherwithPluronicF-127(0.01%w/vfinal;MolecularProbes)for45minat37°C.Afterwashingtwice,each2x106cellsamplewasresuspendedin0.5mlpertubeandprewarmedto37°Cfor10minjustbeforeanalysis.Datawerecollectedfor30stoestablishthebaselineviolet/blue(405:485nm)r...
医源资料库;在线期刊;美国病理学杂志;2003年第162卷第1期PositiveSelectionofAnti–Thy-1AutoreactiveB-1CellsandNaturalSerumAutoantibodyProductionIndependentfromBoneMarrowBCellDevelopment
...endedat5x106/mlandloadedwith8µMIndo-1a.m.()togetherwithPluronicF-127(0.01%w/vfinal;MolecularProbes)for45minat37°C.Afterwashingtwice,each2x106cellsamplewasresuspendedin0.5mlpertubeandprewarmedto37°Cfor10minjustbeforeanalysis.Datawerecollectedfor30stoestablishthebaselineviolet/blue(405:485nm)r...
医源资料库;在线期刊;实验医学杂志;2003年第197卷第1期NovelG423SMutationofHuman7NicotinicReceptorPromotesAgonist-InducedDesensitizationbyaProteinKinaseC-DependentMechanism
...ectedandthenwashedthreetimeswithlysisbuffer.AftermixingwithLaemmlisolution(0.01%bromphenolblue,50mMsodiumphosphate,50%glycerol,and10%SDS)plus7.5%(±)-dithiothreitol,proteinsweredenaturedinboilingwaterfor5min,separatedbySDS-polyacrylamidegelelectrophoresisandtransferredontoapolyvinylidenefluori...
医源资料库;在线期刊;分子药理学杂志;2007年第69卷第3期ARoleforSerotonin(-HT)inHepaticStellateCellFunctionandLiverFibrosis
...espectively.HSCswereleftfor24hoursbeforebeingplacedinlow-serumgrowthmedium(0.01%)overnight.HSCswerethenwashedtwicewithwarmPBS(37??C)andincubatedwith5-hydroxy5-HTtakenupper106cellsperminute.Determinationof5-HTConcentrationinHSCGrowthMediumDay-7culture-activatedratHSCswerewashedtwicewithDulbecco??smod...
医源资料库;在线期刊;美国病理学杂志;2006年第168卷第9期Renal-associatedTLR2mediatesischemia/reperfusioninjuryinthekidney
...roughttoaconcentrationof4x106cells/mlFACSbuffer(PBSsupplementedwith0.5%BSA,0.01%NaN3,and100mMEDTA)andstainedforTLR2(IgG1anti-TLR2mAbnamedT2.5;ref.60)andasecondarygoatanti-mouseIgG1-FITC(SouthernBiotech)for30minutesat4°C.TLR2wasanalyzedonforward-andside-anglescatter–gatedneutrophilsandmonocyteskno...
医源资料库;在线期刊;临床研究杂志;2005年第115卷第10期椎管内麻醉和镇痛的意外及并发症防范
...吸复合麻醉,手术3.5h,术后硬膜外连续输注0.2%罗哌卡因+0.01%吗啡2ml/h镇痛。在接镇痛泵之前给予0.2%布比卡因5ml(含吗啡2mg)作为负荷剂量。病人于30h出现腹腔引流液多,36h(第二天深夜)呼吸困难,紫绀。抢救无效死亡。思考...
合作平台;医学论文;外科论文;麻醉学N-TerminalResiduesControlProteasomalDegradationofRGS2,RGS4,andRGS5inHumanEmbryonicKidney293Cells
...a2+Fluorescence.Themediumwasaspiratedandcellsloadedwith5µMfluo-4AMand0.01%(v/v)PluronicF-127(20%solutionindimethylsulfoxide)inaloadingbufferconsistingofHank‘sbasalsalinesolutionsupplementedwith20mMHEPES,pH7.4,1mMCa2+(ascalciumchloride),1mMMg2+(asmagnesiumchloride),and2.5mMprobenecid.Thecells...
医源资料库;在线期刊;分子药理学杂志;2007年第69卷第4期