GenomicApproachtoIdentificationofMycobacteriumbovisDiagnosticAntigensinCattle
...reappliedtoaHitrapQcolumninasolutioncontaining25mMHEPES(pH7.0),10%glycerol,0.01%Tween20(forTB9.9)orin20mMbis-Tris(pH6.0),1mMEDTA,0.1%Tween20,10%glycerol,and3Murea(forTB27.4).A0to0.5MlineargradientofNaClwasusedforelutionofboundproteins.Fractionswereanalyzedbysilver-stainedSDS-PAGEandbyimmunoblotswith...
医源资料库;在线期刊;微生物临床杂志;2003年第41卷第8期AnimmunoglobulinC-reactivesinglechainantibodyfusionproteininducestolerancethroughreceptoreditinginanormalpolyclonalimmunesystem
...tedusingPBS,0.5mMEDTA,washedtwice,andresuspendedinstainingbuffer(PBS,1%BSA,0.01%NaN3).Cellswereincubatedwithbiotinmouseanti–ratIgG1(BDBiosciences),whichreactswiththelinkerregionofthemacroselfantigen,ormouseIgG2bmonoclonalantibodycarryingorLchains,followedbybiotin-coupledratanti–mouseIgG2a(BDBios...
医源资料库;在线期刊;实验医学杂志;2005年第201卷第5期p38MAPK/HSP25signalingmediatescadmium-inducedcontractionofmesangialcellsandrenalglomeruli
...arvestedin50μlofbufferA(62.5mMTris・HCl,pH6.8,2%SDS,10%glycerol,200mMDTT,0.01%bromophenolblue,1xproteaseinhibitormix),andproteinswereseparatedbySDS-PAGEfollowedbyWesternblotanalysis.Detectionofphosphorylated(activated)MAPKswasperformedusinganti-phospho-Erk1/2monoclonalantibody(Calbiochem),rabbitan...
医源资料库;在线期刊;美国生理学杂志;2005年第288卷第6期LocalInsP3-dependentperinuclearCa2+signalingincardiacmyocyteexcitation-transcriptioncoupling
...ionofCa2+indicatorinthissmallvolumecanbeimpossibletoseeusingopticalmethods(0.01%changeinsignal),althoughitphysicallymustoccur.CaMandproteinkinasesareinvolvedinET-1–inducedHDAC5export.WealsoexploreddownstreamlinksbetweenCa2+releasefromInsP3RandHDACphosphorylation.CaMandCaMKIIassociatewiththeInsP3R(...
医源资料库;在线期刊;临床研究杂志;2006年第116卷第3期ModulationofpMitogen-ActivatedProteinKinaseCascadeandMetalloproteinaseActivityinDiaphragmMuscleinResponsetoFreeRadicalScavengerAdministrati
...edfor5minutesat100??Cinreducingbuffer(50µlofSDSbuffercontaining5%SDS,0.01%bromphenolblue,10%glycerol,and5%ß-mercaptoethanol).ProteinextractswereanalyzedinduplicatebySDS-polyacrylamidegelelectrophoresis(3to10%or5to15%)withprestainedstandardproteins(Bio-Rad,Hercules,CA)toachieveamoreaccura...
医源资料库;在线期刊;美国病理学杂志;2007年第169卷第2期InvivodepletionoflungCD11c+dendriticcellsduringallergenchallengeabrogatesthecharacteristicfeaturesofasthma
...ungsectionswerefixedinacetone,andendogenicperoxidasewasblockedwith0.1%SoAz/0.01%H2O2inPBSfor30min,blockedwithNGSandNRS,andincubatedwithhamsteranti-CD11cfollowedbygoatanti–ArmenianhamsterPO(JacksonImmunoResearchLaboratories);tostainforCD4,sectionswereincubatedwithratanti–mouseCD4-FITC(BDBioscienc...
医源资料库;在线期刊;实验医学杂志;2005年第201卷第6期ApigeninInhibitsImmunostimulatoryFunctionofDendriticCells:ImplicationofImmunotherapeuticAdjuvant
...e,MA).90%.StimulationofDCbyApigenin.ApigeninwasdissolvedinDMSO,orDMSOalone[0.01%(v/v)]wasaddedtoculturesofisolatedDCinsix-wellplates(106cells/ml;3ml/well).DMSO(0.1%)alonewasusedascontrol,becauseofnocytotoxicityinDC.Fortheanalysisofapoptosis,DCwerestimulatedwithLPSorleftwithoutanystimuli,andapoptosis...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第9期ANaturalCYP2B6TATABoxPolymorphism(eC82TC)LeadingtoEnhancedTranscriptionandRelocationoftheTranscriptionalStartSite
...acrylamidegels(0.5xTrisborate-EDTA);gelandrunningbuffercontained4mMMgCl2and0.01%NP-40.Runningconditionswerethesameasdescribedabove.Afterelectrophoresis,gelsweredriedandradioactiveprobevisualizedusingimagingplatesMS2325andaBAS-1800IIplatereader(Fuji).StatisticalAnalyses.Becausesomedatasetswerenotnorm...
医源资料库;在线期刊;分子药理学杂志;2005年第67卷第5期CharacterizationoftheregulationofrenalNa+/H+exchangerNHE3byinsulin
...;HCl,pH6.8,10%glycerol(vol/vol),1%-mercaptoethanol(wt/vol),0.1%SDS(wt/vol),0.01%bromophenolblue(wt/vol)],resolvedbySDS-PAGE,andtransferredtoapolyvinylidenefluoride(PVDF)membrane.Immunoblottingwasperformedwiththeappropriateantiseraata1:1,000dilutionanddevelopedusingenhancedchemiluminescence(Amersham,...
医源资料库;在线期刊;美国生理学杂志;2007年第290卷第2期Protectionoftransplant-inducedrenalischemia-reperfusioninjurywithcarbonmonoxide
...dwithGelvatol,awater-solublemountingmedia(23gpolyvinylalcohol,50mlglycerol,0.01%sodiumazidein100mlPBS),andvisualizedwithanOlympusBX51epifluorescencemicroscopeanddigitizedwithanOlympuscolorvideocamera.TEM.Kidneyswereperfusion-fixedviatheabdominalaortawith2.5%glutaraldehyde,thenstoredovernightinfixati...
医源资料库;在线期刊;美国生理学杂志;2004年第287卷第11期