Low-dosecarbonmonoxideinhalationpreventsdevelopmentofchronicallograftnephropathy
...hGelvatol,awater-solublemountingmedia(23gofpolyvinylalcohol,50mlofglycerol,0.01%sodiumazidein100mlofPBS),visualizedwithanOlympusBX51epifluorescencemicroscope,anddigitizedwithanOlympuscolorvideocamera.Measurementofkidneygraftcorticalbloodflowandarterialbloodpressure.Renalcorticaltissuebloodflowwasmea...
医源资料库;在线期刊;美国生理学杂志;2006年第289卷第3期CaveolinRegulatesKv1.5TraffickingtoCholesterol-RichMembraneMicrodomains
...sterolinserum-freemediaat37°C,withshakingevery5min.Serum-freemediumplus0.01%ethanolwasusedasavehiclecontrol.CholesterollevelswereassayedusingtheAmplexRedCholesterolkitfromInvitrogen(Carlsbad,CA)Cellswerehomogenizedandlysedinreactionbuffercontaining5mMsodiumcholate,and0.1%TritonX-100inphosphate-b...
医源资料库;在线期刊;分子药理学杂志;2008年第70卷第3期CholesterylEsterTransferProteinDecreasesHigh-DensityLipoproteinandSeverelyAggravatesAtherosclerosisinAPOE3-LeidenMice
...ebuffer(0.125mol/LTris,pH6.8;4%)SDS,20%glycerol;10%ß-mercaptoethanol;0.01%bromophenolblue).Sampleswerethenappliedontoa4%to20%TrisGlycineprecastpolyacrylamideminigel(GradiporeLtd,FrenchForest,Australia).Electrophoresiswasperformedaccordingtomanufacturer?sinstructions.Proteinbandswerestainedwith...
医源资料库;在线期刊;动脉硬化血栓血管生物学杂志;2006年第26卷第11期Poly(ADP-ribose)polymerase-1geneablationprotectsmicefromischemicrenalinjury
...asaddedto500μlof56mMHEPESbuffer(pH7.5)containing28mMKCl,28mMNaCl,2mMMgCl2,0.01%digitonin,125nmolofNAD,and0.25μCiof[adenosine-2,8-3H]NAD(specificactivity,3.8Ci/mmol)andincubatedfor5minat37°C.Thereactionwasterminatedbytheadditionof200μlof50%ice-coldtrichloroaceticacid(TCA)andcentrifugation.Thepell...
医源资料库;在线期刊;美国生理学杂志;2005年第288卷第2期TranscriptionalRegulationofMouseeOpioidReceptorGene:Sp3Isoforms(M1,M2)FunctionasRepressorsinNeuronalCellstoRegulatetheeOpioidReceptorGen
...salmonspermDNA.Preclearedlysate(100e蘬)dilutedinimmunoprecipitationbuffer(0.01%SDS,1.1%TritonX-100,1.2mMEDTA,16.7mMTris·HCl,pH8.1,and167mMNaCl)wasusedforovernightimmunoprecipitationwith10e蘥ofSp3andHA(Covance,Richmond,CA)antibodyat4°C.Complexeswerecollectedfor4hbyusingrecombinantproteinA-agarose...
医源资料库;在线期刊;分子药理学杂志;2005年第67卷第5期SomatostatinIncreasesPhospholipaseDActivityandPhosphatidylinositol4,5-bisphosphateSynthesisinClonalCellsHIT-T15
...Sfor1hatroomtemperature.TheprimaryPLDantibodieswerediluted1:30inwashbuffer(0.01%Tween20inPBS)andincubatedwiththePVDFmembranesfor1hatroomtemperature.Theblotswerewashedthreetimesfor10mineachwithwashbuffer.Thesecondaryantibody(goatanti-rabbitIgGconjugatedtohorseradishperoxidase;Pierce,Rockford,IL)wasdi...
医源资料库;在线期刊;分子药理学杂志;2005年第67卷第6期AcuteandchronicchangesincholesterolmodulateNa-PicotransportactivityinOKcells
...on.Thefitswereacceptedwhentwoconsecutiveiterationschangedthesumofsquaresby<0.01%.Asanindicationofthegoodnessofthefits,thecorrelationcoefficient(r)anddegreesoffreedomareshown(degreesoffreedomstatethenumberofdatapointsminusthenumberoffittedparameters).Thesignificanceofthedifferencesamongthefitswascalc...
医源资料库;在线期刊;美国生理学杂志;2005年第288卷第7期ofFibulin-ExpressionIsAssociatedwithBreastCancerProgression
...edwith1xSSC/0.1%sodiumdodecylsulfatefor5minutes,followedbyawashwith0.1xSSC/0.01%sodiumdodecylsulfate,and0.1xSSC.Thearraysweredriedbycentrifugationfor5minutesat1000rpmandscannedwithaScanArray4000confocallasersystem(Perkin-Elmer,Wellesley,MA).ImageanalysiswasperformedbytheQuantArraysoftwarepackage(Per...
医源资料库;在线期刊;美国病理学杂志;2007年第169卷第5期ExpressionofP2XandP2Yreceptorsintheintramuralparasympatheticgangliaofthecaturinarybladder
...d,CA)ofthefollowingcomposition(inmM):170NaCl,7KCl,1.6Na2HPO4,6D-glucose,and0.01%phenolred,pH7.3(Invitrogen).Underadissectingmicroscope,theintramuralbladdergangliawerecutintosmallersectionswithsterilescissorsandtransferredintopreactivated(37°Cfor10min)membranefilter-sterilized(0.2µm2)HBSSso...
医源资料库;在线期刊;美国生理学杂志;2006年第289卷第5期Microarray-BasedDetectionof90AntibioticResistanceGenesofGram-PositiveBacteria
...incubatedfor15additionalminutesat30°Cand550rpm.Thearrayswerewashedin2xSSC-0.01%TritonX-100at30°Cfor5minandin2xSSCandthen0.2xSSCfor5minat20°C.Thearrayswerekeptat20°Cinthelastwashingsolutionuntilvisualization.Thehybridizedprobeswereenhancedusing100μloftetramethylbenzidineperoxidasesubstrate(Clond...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第5期