ANaphthoquinoneDerivative,Shikonin,HasInsulin-LikeActionsbyInhibitingBothPhosphataseandTensinHomologDeletedonChromosome10andTyrosinePhosph
...ininthepresenceorabsenceof5mMphenylphosphateinTris-bufferedsalinecontaining0.01%bovineserumalbuminand1Mdithiothreitol.Themixturewasdiluted100-foldwithareactionbufferconsistingof100mMsodiumacetate,pH5.5,100mMNaCl,1mMdithiothreitol,and5mMpNPP.Thephosphatasereactionwasallowedtoproceedfor10minandwasstop...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第9期StimulationofrenalsulfatesecretionbymetabolicacidosisrequiresNa+/H+exchangeinductionandcarbonicanhydrase
...ckingbuffer[PBScontaining0.05%polyoxyethylene-sorbitanmonolaurate(Tween20),0.01%antifoamA,0.02%NaN3,and10%nonfatdrymilk]overnightat4°Cfollowedby1-hincubationatroomtemperaturewithprimaryantibodiesdiluted1:500inblockingbuffer.Primaryantibodiesincludedmouseanti-porcineNHE1(MAB3140,ChemiconInternationa...
医源资料库;在线期刊;美国生理学杂志;2005年第288卷第7期AngiotensinII-inducedcalciumsignalingintheafferentarteriolefromratswithtwo-kidney,one-cliphypertension
...dkidneys(P0.1).Thekidneyweight-to-bodyweightratioincontrolrats(0.35±0.01%,n=10)washighercomparedwiththeratiofortheclippedkidney(0.24±0.02%,n=10,P<0.005).Thekidneyweight-to-bodyweightratiofornonclippedkidneysinthe2K1Crats(0.43±0.04%,n=10)wasincreasedcomparedwiththeratioforcontrol...
医源资料库;在线期刊;美国生理学杂志;2006年第289卷第7期LipoxinARegulatesBronchialEpithelialCellResponsestoAcidInjury
...,30minutes)influorescence-activatedcellsortingbuffer(DPBS+5%fetalcalfserum+0.01%sodiumazide)with1mgofrabbitanti-humanALXorwithclass-matchedirrelevantrabbitIgG1.Eachsamplewaswashed,resuspendedinfluorescence-activatedcellsortingbuffercontainingafluoresceinisothiocyanate-conjugatedgoatanti-rabbitIgGant...
医源资料库;在线期刊;美国病理学杂志;2006年第168卷第4期EffectsofchloridechannelblockersonratrenalvascularresponsestoangiotensinIIandnorepinephrine
...ura2-AMfor45-60mininthedarkatroomtemperature.Fura2loadingwasfacilitatedwith0.01%PluronicF-127(Sigma).A5-µlmicropipettewasusedtotransferthevesseltoaPSS-containingchamberonthestageofaninvertedmicroscope(Olympus).Theendsofthevesselwereaspiratedintoglassholdingpipettestoobtainmechanicalstability.M...
医源资料库;在线期刊;美国生理学杂志;2004年第287卷第2期NeuroprotectiveEffectsof17-EstradiolandNonfeminizingEstrogensagainstH2O2ToxicityinHumanNeuroblastomaSK-N-SHCells
...LittleChalfont,Buckinghamshire,UK).ThreemillilitersofHBSScontainingvehicle(0.01%ethanol,estrogens,orICI182,780)wasaddedintothechamber.TreatmentwithH2O2wascarriedoutbyaddingtheappropriateconcentrationsintothechamber.Theexcitationwavelengthwasalternatedbetween340and380nm,andemissionfluorescencewasreco...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第7期Macrophage-SpecificExpressionofHumanLysosomalAcidLipaseCorrectsInflammationandPathogenicPhenotypesinlalC/CMice
...5µlofdilutedliverextract,and125µlofassaybuffer(0.2mol/LNa2Acand0.01%Tween80,pH5.5)at37??Cfor30minutes.Thereactionwasstoppedbyadding100µlof0.75mol/LTris,pH8.0.Theresultingfluorescencesignalwasdetectedatexcitation360nmandemission460nm.Therelativefluorescenceunitsfromeachsampleweredir...
医源资料库;在线期刊;美国病理学杂志;2006年第168卷第9期RoleoftheforkheadproteinFoxO1in?cellcompensationtoinsulinresistance
...ting?cells,asmeasuredbyKi67incorporation,wererareinWT,307,L1,andL1/307mice(0.01%of?cells)butwerecompletelyabsentin305andL1/305mice,indicatingthatproliferationwaseffectivelypreventedbytheFoxO1transgene.Itshouldbenotedthat,althoughL1/305micehadsmaller?cellmassthanL1/307mice,theircirculatinginsulinleve...
医源资料库;在线期刊;临床研究杂志;2006年第116卷第3期Sodiumandpotassiumhandlingbythealdosterone-sensitivedistalnephron:thepivotalroleofthedistalandconnectingtubule
...egrownormallyonahigh-sodiumdiet(0.3%)butcannottolerateavery-low-sodiumdiet(0.01%),whichtriggersarapidandmarkedsalt-losingsyndrome(72).TheseresultsshowthatevenintheabsenceofanyENaCactivityintheCCD,micecanstillappropriatelyincreaserenalsodiumreabsorption,whichstressestheimportanceofthelateDCTandtheCNT...
医源资料库;在线期刊;美国生理学杂志;2004年第287卷第10期ConstitutiveActivationandEnvironmentalChemicalInductionoftheArylHydrocarbonReceptor/TranscriptionFactorinActivatedHumanBLymphocytes
...iledfor5minin1xSDS-PAGEsamplebuffer(50mMTrisbuffer,pH6.8,10%glycerol,2%SDS,0.01%bromphenolblue,and1%-mercaptoethanol)beforeSDS-PAGEelectrophoresisthrougha6.8%polyacrylamidegelandovernighttransferontoanitrocellulosemembrane(Bio-Rad).Aftertransfer,membraneswereblockedwith5%skimmilkpowderin1xTris-buffe...
医源资料库;在线期刊;分子药理学杂志;2005年第67卷第5期