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UseofPooledUrineSamplesandAutomatedDNAIsolationToAchieveImprovedSensitivityandCost-EffectivenessofLarge-ScaleTestingforChlamydiatrachomati
...eningforChlamydiatrachomatisdependsontheavailabilityofnoninvasivesamples,lowcosts,andhigh-qualitytesting.ToevaluateC.trachomatistestingwithpregnantwomen,first-voidurinespecimensfrom750consecutiveasymptomaticpregnantwomenfromtheRotterdamarea(TheNetherlands)werecollected.Initially,weinvestigatedtheper...
医源资料库；在线期刊；微生物临床杂志；2005年第43卷第9期 -
EnhancedEnzymeImmunoassaywithNegative-Gray-ZoneTestingComparedtoaSingleNucleicAcidAmplificationTechniqueforCommunity-BasedChlamydialScreening
TheMilneCentre,UnitedBristolHealthcareNHSTrust,BristolBS28HWDepartmentofPathologyandMicrobiology,UniversityofBristol,BristolRoyalShrewsburyHospitalTrust,ShrewsburyPublicHealthLaboratoryServiceBristolLaboratory,BristolBS28ELHealthProtectionAgency,MyrtleRoad,BristolBS28ELDepartmentofSocialMedicine,Uni...
医源资料库；在线期刊；微生物临床杂志；2005年第43卷第5期 -
IdentificationofpoxvirusCD8+Tcelldeterminantstoenablerationaldesignandcharacterizationofsmallpoxvaccines
...D8+Tcellsandgreatlyimpededdevelopmentofmousesmallpoxvaccinationmodels.Here,weuseavacciniavirus(VACV)expressionlibrarycontainingeachofthepredicted258openreadingframestoidentifyfivepeptidedeterminantsthataccountforapproximatelyhalfoftheVACV-specificCD8+TcellresponseinC57BL/6mice.Weshowthattheprimaryim...
医源资料库；在线期刊；实验医学杂志；2005年第201卷第1期 -
IncreasedRateofDNARecoveryfromUnitedKingdomEpidemicClostridiumdifficilePCRRibotype1StrainsStoredCryogenically
...mentofMicrobiology,LeedsTeachingHospitals,LeedsLS13EX,UnitedKingdomABSTRACTWenotedthatsomeClostridiumdifficileisolatesarenonrecoverableafterfrozenstorageandsousedmoleculartypinganalysistocharacterizeDNAfromthesestrains.TherecoveryrateofC.difficilePCRribotype1wasstatisticallysignificantlygreaterthant...
医源资料库；在线期刊；微生物临床杂志；2005年第43卷第11期 -
UseofVariable-NumberTandem-RepeatTypingToDifferentiateMycobacteriumtuberculosisBeijingFamilyIsolatesfromHongKongandComparisonwithIS6110Rest
...rculosisBeijingfamilyisolatesmaycausemorethanaquarterofalltuberculosiscasesworldwide,areemerginginsomeareas,andareoftenassociatedwithdrugresistance.Earlyrecognitionoftransmissionofthisgenotypeisthereforeimportant.Toevaluatetheusefulnessofvariable-numbertandem-repeat(VNTR)typingtodiscriminateandrecog...
医源资料库；在线期刊；微生物临床杂志；2005年第43卷第1期 -
突变型α-核突触蛋白的自噬性降解途径及可能机制
...蛋白的表达和超氧化物歧化酶（SOD）的水平。结果（1）WesternBlot法检测α-核突触蛋白的表达：A30P＋渥曼青霉素组（A30P＋W组）、A30P＋1-甲基-±苯基吡啶组（A30P＋MPP^＋组）较A30P组明显增高，以A30P＋W组最为明显；而A30P＋雷帕霉素...
行业资讯；临床快报；神经科 -
Optimizationofaselfantigenforpresentationofmultipleepitopesincancerimmunity
1SwimAcrossAmericaLaboratoryofTumorImmunology,MemorialSloan-KetteringCancerCenter,NewYork,NewYork,USA.2WeillMedicalandGraduateSchoolsofCornellUniversity,NewYork,NewYork,USA.AbstractTcellsrecognizingselfantigensexpressedbycancercellsareprevalentintheimmunerepertoire.However,activationoftheseautoreact...
医源资料库；在线期刊；临床研究杂志；2006年第116卷第5期 -
DetectionofHumanInfluenzaAVirusesbyLoop-MediatedIsothermalAmplification
...tyofHongKongDepartmentofMicrobiology,QueenMaryHospital,HongKongABSTRACTHerewedescribetheuseoftheloop-mediatedisothermalamplification(LAMP)methodtodetecthumaninfluenzaviruses(H1toH3).Ourresultswerecorrelated100%withresultsdeducedfromroutineclinicaldiagnostictests.Inaddition,wealsodevelopedaLAMPassays...
医源资料库；在线期刊；微生物临床杂志；2005年第43卷第1期 -
Enhancementofclonogenicityofhumanmultiplemyelomabydendriticcells
...apy2LaboratoryofCellularPhysiologyandImmunology,TheRockefellerUniversity,NewYork,NY100213St.Vincent‘sCancerCenter,NewYork,NY100214HematologyService,MemorialSloanKetteringCancerCenter,NewYork,NY10021Infiltrationbydendriticcells(DCs)isacommonfeatureofmosthumantumors.Priorstudiesevaluatingtheinteract...
医源资料库；在线期刊；实验医学杂志；2006年第203卷第8期 -
利多卡因和异丙酚预先给药对哮喘大鼠肺组织NO和内皮素水平的影响
...势。中华麻醉学杂志三月第三期刊登一项研究，研究者将Wistar大鼠32只,随机分为4组（n=8）：对照组（Ⅰ组）、哮喘组（Ⅱ组）、异丙酚组（Ⅲ组）和利多卡因组（Ⅳ组）。Ⅱ组、Ⅲ组、Ⅳ组大鼠用卵清蛋白辅以氢氧化铝注射致...
行业资讯；临床快报；麻醉科