NovelMechanismofInhibitionofNuclearFactor-BDNA-BindingActivitybyDiterpenoidsIsolatedfromIsodonrubescens
...Ssamplebuffer(62.5mMTris-HCl,pH6.8,2%SDS,10%glycerol,50mMdithiothreitol,and0.01%bromphenolblue).TheproteinsofinterestweredetectedbyWesternblotanalysisusingtheantibodiesdescribed.ReverseTranscriptase-RealTimePolymeraseChainReactionAssay.TotalRNAwasisolatedusingRNeasyMiniKit(Qiagen,Valencia,CA).Alloft...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第2期TLC显色试剂及配方大全
...3)醛酮类①品红/亚硫酸检出物:醛基化合物。溶液:I.0.01%品红溶液,通入二氧化硫直至无色;Ⅱ.0.05mol/L氯化汞溶液;Ⅲ.O.05mol/L硫酸溶液。方法:将I、Ⅱ、Ⅲ以1:1:10混合,用水稀释至l00ml。②邻联茴香胺检出物:醛...
药品天地;专业药学;实验技术;基本实验技术;常用试剂精制抗毒素制造及检定规程
...氯甲烷使最终含量为0.5%(ml/ml)或硫柳汞液使最终含量为0.01%(g/ml)为防腐剂。用于制造冻干抗毒素者只用三氯甲烷为防腐剂。 1.2.6.4调节pH至6.0~7.0。 1.2.6.5除菌过滤,并做无菌试验。 1.2.7冻干 经除菌过滤及半成品...
医源资料库;医源图书馆;教材类;中国生物制品规程VacuolarLeukoencephalopathywithWidespreadAstrogliosisinMiceLackingTranscriptionFactorNrf
...ledwaterfor2minuteswasfollowedbyimmersioninthestainingsolutionfor20minutes(0.01%stocksolution,4mlofstocksolutiondilutedin96mlof0.1%aceticacid).Afterstaining,slideswerewashedthreetimesindistilledwaterfor1minuteeachandwereallowedtoairdryovernight.Slideswereplacedonawarmerat55??Cfor5minutes,clearedinxy...
医源资料库;在线期刊;美国病理学杂志;2007年第169卷第6期第七节 实验室检查
...mmol/LDTT(二硫基苏糖醇),200μmol/ldNTP,100μmol/L引物(TP7和TP8),0.01%牛血清白蛋白(无DNase和RNase),1UTaqDNA聚合酶,5μ1样品,可加入0.05mmol/L四甲胺化氯增强PCR。 PCR1:先用引物TP7和TP8扩增出617bp片段,反应过程:94℃3min后进入循环过程:94℃1min,6...
医源资料库;医源图书馆;教材类;基因诊断与性传播疾病CCL11(Eotaxin)InducesCCR3-DependentSmoothMuscleCellMigration
...5cellswereincubatedat4°Cfor45minutesinFACSbuffer(PBS,1%bovineserumalbumin,0.01%NaN3)containingmonoclonalanti-mouseCCR3(FAB729F;RDSystems).AnirrelevantIgG2aantibodywasusedasanisotypecontrol.Unboundantibodywasremovedbywashingwith2mLFACSbuffer,andthecellswereresuspendedinthesamebuffercontaininga1:20di...
医源资料库;在线期刊;动脉硬化血栓血管生物学杂志;2004年第24卷第7期AnalysisofGenomicDownsizingontheBasisofRegion-of-DifferencePolymorphismProfilingofMycobacteriumtuberculosisPatientIsolatesRevealsGeographicP
...onmixturecontaining50nMofeachprimer,10mMTris-HCl(pH8.8),50mMKCl,1.5mMMgCl2,0.01%gelatin,10%dimethylsulfoxide,200μMnucleotidemix,1UTaqpolymerase,and1to10ngoftemplateDNA,andthevolumewasmadeupwithsteriledistilledwater.Afterdenaturationat95°Cfor3min,thereactionmixturewasprocessedforanother35cyclesat95...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第12期FibroblastGrowthFactor-2FailedtoInduceAngiogenesisinJunctionalAdhesionMolecule-A-DeficientMice
...containing1%paraformaldehyde/0.2%(wt/vol)glutaraldehyde/0.02%(wt/vol)NP-40/0.01%sodiumdeoxycholatefor1to6hoursat4°C.Afterfixation,slideswerestainedasdescribedpreviously.26RetinalFluoresceinAngiogramMicewereanesthetizedwithketamine/xylazine(3to5mg/kg),andfluoresceinisothiocyanate(FITC)-dextran(FI...
医源资料库;在线期刊;动脉硬化血栓血管生物学杂志;2006年第26卷第9期InfluenceoftheMembraneLipidStructureonSignalProcessingviaGProtein-CoupledReceptors
...gusetal.(1994).Experimentswerecarriedoutasdescribedabove,using5nMGproteins,0.01%Thesit,and2.5nM[35S]GTPSandinthepresenceorabsenceof100eOA,DNM,PE,orCHS.Bindingof[3H]RX821002(20nM)and[3H]UK14304(1nM)to3T3-ARcellmembraneswasperformedinthepresenceorabsenceofOA,EA,SA,DNM,PE,orCHS,for30minonice.Themembran...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第1期附录二 原位杂交组织化学常用试剂及处理
...可反复冻融,无明显影响。用前充分混合。 工作液(0.01%): 充分混合,静止待气泡消失。 (2)明胶液 配法:先称取明胶溶于500~800mlDEPC水中,加热搅拌助溶,待明胶完全溶解以后,加入甲明矾溶解后即可使用...
医源资料库;医源图书馆;教材类;实用免疫细胞与核酸