SequentialexpressionofNKCC2,TonEBP,aldosereductase,andureatransporter-Aindevelopingmousekidney
...idine(DAB)in0.05MTrisbufferfor5min.Then,H2O2wasaddedtoafinalconcentrationof0.01%andtheincubationwascontinuedfor10min.Thesectionswerewashedthreetimeswith0.05MTrisbuffer,dehydratedinagradedseriesofethanol,andembeddedinPoly/Bed812resin(Polysciences,Warrington,PA).Thesectionswereexaminedwithalightmicros...
医源资料库;在线期刊;美国生理学杂志;2007年第290卷第1期第四节 影响PCR的主要因素
...酰胺<5%100 10%82 20%17甲酰胺<10%100 15%86 20%39SDS0.001%105 0.01%10 0.1%<0.1% 用鲱精DNA,70℃,10min内dNTP的掺入量计算,标准条件为100%。 纯9.4KDaTaq酶不含3’→5’核酸外切酶活力。误掺入率取决于dNTP浓度。但Taq酶具有DNA依赖...
医源资料库;医源图书馆;教材类;实用免疫细胞与核酸SensitivityofPCRTargetingtheIS2404InsertionSequenceofMycobacteriumulceransinanAssayUsingPunchBiopsySpecimensforDiagnosisofBuruliUlcer
...atdescribedbyKoxetal.(9),withfinalreactionconcentrationsof10mMTris,50mMKCl,0.01%gelatin,1.5mMMgCl2,0.1%TritonX-100,0.2mM(each)deoxynucleosidediphosphate(dATP,dCTP,dGTP,anddUTP)(RocheDiagnosticsGmbH,Germany),0.2μMeachofforwardprimerPU4F(5‘-GCGCAGATCAACTTCGCGGT3‘)andreverseprimerPU7Rbio(5‘-GCCC...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第8期Effectsofisoenergetichigh-carbohydratecomparedwithhigh-fatdietsonhumancholesterolsynthesisandexpressionofkeyregulatorygenesofcholesterolme
...metabolismDeuteriumenrichmentsinplasmawaterwere0.32±0.02%and0.33±0.01%duringtheHCandHFdiets,respectively.Thecorrespondingenrichmentsinplasmafreecholesterolwere0.45±0.03%and0.38±0.03%,respectively.Theseenrichmentswerehigherthanthoseinplasmawaterbecausetherearemultiplepossibleincor...
医源资料库;在线期刊;美国临床营养学杂志;2001年73卷第5期Measurementofmagnesiumabsorptionandretentionintype2diabeticpatientswiththeuseofstableisotopes
...w(30).Reproducibilityforthe24Mg/25Mgisotoperatio(6independentruns)was±0.01%(relativeSD).DataandstatisticalanalysisApparentmagnesiumabsorptionandretentionfromtheisotopicallylabeledtestmealswerecalculatedonthebasisoftheamountofratioofisotopiclabeltonaturalmagnesiumin6-dfecalpoolsand6-durinarypool...
医源资料库;在线期刊;美国临床营养学杂志;2003年78卷第3期BiologicalSignificanceofDecreasedHSP27inHumanAtherosclerosis
...enCPsandNCPstissuesamples(15mg)wereincubatedinTris/HCl,pH7.4,100mmol/LNaCl,0.01%Tween20withtheplasminsubstrateTosyl-Gly-ProArg-Mca(5µmol/L;Sigma).20Substratehydrolysiswasmonitoredfor2hoursbyspectrofluorometry(HitachiF-2000;excitation347nmemission440nm).Whenindicated,CPwasdividedintosimilarpiec...
医源资料库;在线期刊;动脉硬化血栓血管生物学杂志;2006年第26卷第6期MusclefibertypeIIXatrophyisinvolvedinthelossoffat-freemassinchronicobstructivepulmonarydisease
...odiumacetate/Lbuffer(pH5.6)containing7mmolglucose-1-phosphate/L,1mmolAMP/L,0.01%(wt:vol)glycogen,and15%(vol:vol)ethanol,afterwhichthenewlyformedpolysaccharidewascoloredwithLugol‘siodine(23).COXandSDHcrosssectionsweredehydratedinethanolandmountedinEntallan(Merck,Darmstadt,Germany).GlyPsectionswerem...
医源资料库;在线期刊;美国临床营养学杂志;2002年76卷第1期DifferentialregulationofB/Kproteinexpressioninproximalanddistaltubulesofratkidneyswithischemia-reperfusioninjury
...idinein0.05MTrisbufferfor5min,afterwhichH2O2wasaddedtoafinalconcentrationof0.01%andtheincubationwascontinuedfor10min.Afterbeingwashedwith0.05MTrisbufferthreetimes,thesectionsweredehydratedinagradedseriesofethanolandembeddedinEpon-812.Fromallanimals,50-µm-thickvibratomesectionsthroughtheentirek...
医源资料库;在线期刊;美国生理学杂志;2007年第290卷第1期Postprandialeffectsofdietarytransfattyacidsonapolipoprotein(a)andcholesterylestertransfer
...cidsasoleicacidand9.9%astrans18:1.Bothfatscontainedsmallamountsoftrans16:1(0.01%totalfattyacids)andtrans18:2isomers(0.50%totalfattyacids).Incalculatingtheratiooffattyacidclasses,transfattyacidswereincludedwiththemonounsaturates.Mealswerepreparedbycombininganamountofoilblendcontaining1g/kgfatwith2.96...
医源资料库;在线期刊;美国临床营养学杂志;2003年77卷第5期AdenoviralGeneTransferofFGF-5toHibernatingMyocardiumImprovesFunctionandStimulatesMyocytestoHypertrophyandReentertheCellCycle
...wasstilllessthan1%ofmyocytes(LAD0.29±0.04%andremote0.21±0.03%versus0.03±0.01%inshams).IncreasedKi-67positivitywasnotrestrictedtomyocytesbutwasalsodetectedinfibroblasts,endothelialcells,andsmoothmusclecells.Nonmyocytestainingrepresented51±7%ofallnuclearKi-67staining.AlthoughKi-67reflectsactivecel...
医源资料库;在线期刊;循环研究杂志;2005年第95卷第4期