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ImprovedMolecularDetectionofDengueVirusSerotype1Variants
InstitutPasteurduCambodge,1NationalDengueControlProgramme,MinistryofHealth,2WorldHealthOrganization,PhnomPenh,Cambodia3Received10September2002/Returnedformodification26February2003/Accepted23May2003 ABSTRACTTopAbstractTextReferencesThedenguevirusmoleculartypingmethoddescribedbyLanciottiandcoworker...
医源资料库；在线期刊；微生物临床杂志；2003年第41卷第8期 -
NeurochemicalCharacterizationofaNeuroprotectiveCompoundfromParawixiabistriataSpiderVenomThatInhibitsSynaptosomalUptakeofGABAandGlycine
...ntributionofthisworkistheisolationofaneuroprotectivecompoundreferredtoas2-amino-5-ureidopentanamide(FrPbAII)(Mr=174)fromParawixiabistriataspidervenomandaninvestigationofitsmodeofaction.FrPbAIIinhibitssynaptosomalGABAuptakeinadose-dependentmannerandprobablydoesnotactonNa+,K+,andCa2+channels,GABABrece...
医源资料库；在线期刊；分子药理学杂志；2006年第68卷第6期 -
EfficientDiscriminationwithinaCorynebacteriumdiphtheriaeEpidemicClonalGroupbyaNovelMacroarray-BasedMethod
...ectrepeat(DR)loci39kbdownstreamand180kbupstreamoftheoriCregion,consistingofminisatellite(27-to36-bp)alternatingDRsandvariablespacers.WedesignatedtheselociDRAandDRB,respectively.Areverse-hybridizationmacroarray-basedmethodhasbeendevelopedtostudypolymorphism(thepresenceorabsenceof21differentspacers)in...
医源资料库；在线期刊；微生物临床杂志；2005年第43卷第4期 -
TransitionfromIntravenousEpoprostenoltoIntravenousTreprostinilinPulmonaryHypertension
...noisDukeUniversityMedicalCenter,Durham,NorthCarolinaUniversityofAlabama,Birmingham,AlabamaUniversityofMichigan,AnnArbor,MichiganColumbiaUniversityCollegeofPhysiciansandSurgeons,NewYork,NewYorkABSTRACTRationale:Intravenousepoprostenolimprovesexercisecapacityandsurvivalinpatientswithpulmonaryarterialh...
医源资料库；在线期刊；美国呼吸和危急护理医学；2005年第171卷第12期 -
人活化血小板单抗SZ-51单链抗体的基因构建及高效表达
...=0.6时，加IPTG（0.7mmol/L）诱导，30℃振摇2.5h，在4℃下5000r/min离心10min收获细菌。以20mmol/LTris-HCl，0.15mol/LNaCl洗涤液洗1次，加1/10（v/v）溶菌液（20mmolTris-HCl，含1%TritonX-100，250μmol/LPMSF以及100μg/ml溶菌酶），30℃放置15min，然后在冰上...
合作平台；医学论文；临床医学与专科论文；检验医学 -
双侧V-Y推移肌皮瓣修复骶尾部褥疮的临床应用
...括骶骨棘突、髂后上棘等均应咬除并修整，用碘伏浸泡10min，并用生理盐水冲净。 1.2.2皮瓣修复褥疮 在骶尾部创面左右两侧设计三角形皮瓣，在设计皮瓣时，将底边位于内侧，距创缘约1～2cm，不与创面相连，大小与创面...
医源资料库；在线期刊；中华现代临床医学杂志；2010年第8卷第9期 -
RoleofChemR23indirectingthemigrationofmyeloidandplasmacytoiddendriticcellstolymphoidorgansandinflamedskin
...Langerhanscellswerenegative.Chemerinexpressionwasselectivelydetectedontheluminalsideofhighendothelialvenulesinsecondarylymphoidorgansandindermalendothelialvesselsoflupuserythematosusskinlesions.Chemerin+endothelialcellsweresurroundedbyChemR23+plasmacytoidDCs.Thus,ChemR23isexpressedandfunctionalinpla...
医源资料库；在线期刊；实验医学杂志；2005年第201卷第4期 -
InvolvementofDopamineSysteminRegulationofNa+,K+-ATPaseintheStriatumuponActivationofOpioidReceptorsbyMorphine
...nadose-dependentmanner.ThisactioncouldbesignificantlyinhibitedbyD2-likedopaminereceptorantagonistS(-)-3-chloro-5-ethyl-N-[(1-ethyl-2-pyrrolidinyl)methyl]-6-hydroxy-2-methoxybenzamine(eticlopride).Contrarytoshorttermmorphinetreatment,long-termmorphinetreatmentsignificantlysuppressedNa+,K+-ATPaseactiv...
医源资料库；在线期刊；分子药理学杂志；2007年第69卷第2期 -
TheBioreductionofaSeriesofBenzoquinoneAnsamycinsbyNAD(P)H:QuinoneOxidoreductase1toMorePotentHeatShockProtein90Inhibitors,theHydroquinone
...NAD(P)H:quinoneoxidoreductase1(NQO1)inthebioreductivemetabolismof17-(allylamino)-demethoxygeldanamycin(17AAG)tothecorrespondinghydroquinone,amorepotent90-kDaheatshockprotein(Hsp90)inhibitor.Here,wereportanextensivestudywithaseriesofbenzoquinoneansamycins,whichincludesgel-danamycin,17-(amino)-17-deme...
医源资料库；在线期刊；分子药理学杂志；2006年第68卷第10期 -
EvaluationofPCRforDiagnosisofIndianKala-AzarandAssessmentofCure
...ninathermalcycler(MJResearch)programmedfor40cyclesofdenaturationat94°Cfor1min,annealingat45°Cfor1min,andextensionat72°Cfor2min,whichwereprecededbyinitialdenaturationat94°Cfor2min.Finalextensionwasfor3minat72°C.Theproductwasanalyzedbyelectrophoresisina1%agarosegelcontainingethidiumbromide(0.5μg...
医源资料库；在线期刊；微生物临床杂志；2005年第43卷第7期