Long-termtreatmentwithcyclosporinedecreasesaquaporinsandureatransportersintheratkidney
...-diaminobenzidine(DAB)inTBfor5min.TissuesectionswerethenincubatedwithH2O2(0.01%)for10min.ThetissuesectionswerewashedthreetimeswithTB,dehydratedinagradedseriesofethanol,andembeddedinpoly/Bed812resin(Polysciences,Warrington,CA).ThetissuesectionswereexaminedwithlightmicroscopyandphotographedwithanOlymp...
医源资料库;在线期刊;美国生理学杂志;2004年第287卷第7期癌的靶向基因治疗基础
...形成试验和体内的脾集落检定.例如小鼠骨髓瘤中只有1%~0.01%的细胞能在软琼酯中形成集落,同样肺癌、卵巢癌或神经母细胞瘤在软琼酯中具集落形成能力的也只有0.1%~0.02%的比例.白血病细胞也只有1~4%的细胞在体内能形成脾集...
行业资讯;临床快报;肿瘤相关罗格列酮改善高脂饲养大鼠胰岛素抵抗机制的研究
...赖氨酸的载玻片上,37℃烤30min,用4%多聚甲醛固定30min,0.01%TritonX-100-PBS洗3×3min,再用0.4%胃蛋白酶消化30min,PBS洗3×3min,加TUNEL混合液,37℃×1h,PBS洗3×3min,甘油封片,荧光显微镜下观察。观察后再PBS洗3×3min,滴加抗FITC二抗(...
医源资料库;在线期刊;中华现代内科学杂志;2006年第3卷第7期1例妊娠合并侵蚀性葡萄胎患者的整体护理
...娠合并葡萄胎极为少见,其发生率约大约是妊娠的0.005%~0.01%[1],妊娠合并侵蚀性葡萄胎临床上更为罕见。2008年3月四川省人民医院产科收治1例中期妊娠合并侵蚀性葡萄胎,中央性前置胎盘,妊高症,贫血,低蛋白血症患者,...
医源资料库;在线期刊;中华现代护理学杂志;2009年第6卷第10期肝干细胞与干细胞移植治疗急慢性肝病的研究进展
...mesenchymalstemcells,MSCs)。骨髓中MSCs的含量很低,约为0.001%~0.01%,因此应用MSCs前,需对其进行分离及体外培养扩增。目前常用的分离方法为贴壁筛选法、密度梯度离心法、流式细胞仪分选法和免疫磁珠分离法,其中流式细胞仪分选...
医源资料库;在线期刊;中华现代临床医学杂志;2006年第4卷第6期ArecytokinegenepolymorphismsassociatedwithoutcomeinpatientswithidiopathicintermediateuveitisintheUnitedKingdom?
...esconsistedof67mMTRISbasepH8.8,16.6mMammoniumsulphate,2mMmagnesiumchloride,0.01%(v/v)Tween20,200μMofeachdNTP,1–4μMofeachallelespecificprimer,0.1μMofcontrolprimer,0.1μgofDNA,and0.1875unitsofTaqPolymerase(Abgene,Epsom,UK).AllreactionswerecarriedoutinaGeneampPCRSystem9700thermalcycler(PEBiosystem...
医源资料库;在线期刊;英国眼科学杂志;2005年第89卷第8期ClonalSpreadofaClostridiumdifficileStrainwithaCompleteSetofToxinA,ToxinB,andBinaryToxinGenesamongPolishPatientswithClostridiumdiffici
...saddedtoaPCRmixture(50μl)containing10mMTris-HCl(pH9.0),50mMKCl,2.5mMMgCl2,0.01%gelatin(wt/vol),0.1%TritonX-100(vol/vol),0.2mMconcentrationsofeachofthefourdeoxyribonucleotidetriphosphates(AmershamBiosciences,LittleChalfont,Buckinghamshire,UnitedKingdom),1.2UofSuperTaqDNApolymerase(HTBiotechnology,Ca...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第1期HowSensitiveIstheAssociationbetweenOzoneandDailyDeathstoControlforTemperature
...mhourlyozoneconcentrationswasassociatedwitha0.23%(95%confidenceinterval[CI]0.01%,0.44%)increaseintheriskofdying.Thisfindingwasindistinguishablefromtheriskwhenonlymatchingonseasonandcontrollingfortemperaturewithregressionsplines(0.19%;95%CI03%,0.35%).Controlforsuspendedparticulatematterwithanaerodyna...
医源资料库;在线期刊;美国呼吸和危急护理医学;2005年第171卷第3期ActivationoftheAngiotensinIIType1ReceptorLeadstoMovementoftheSixthTransmembraneDomain:AnalysisbytheSubstitutedCysteineAccessibilityMetho
...ndresuspendedinbindingbuffer(25mMTris-HCl,pH7.4,100mMNaCl,5mMMgCl2,0.1%BSA,0.01%bacitracin,0.01%soybeantrypsininhibitor).Saturationbindingexperimentsweredonebyincubatingbrokencells(20-40µgofprotein)for1hatroomtemperaturewithincreasingconcentrationsof125I-[Sar1,Ile8]AngIIinafinalvolumeof500&mic...
医源资料库;在线期刊;分子药理学杂志;2007年第69卷第7期神经营养素-3及其受体在小鼠脾的分布和基因表达
...异戊巴比妥钠麻醉,经左心室灌注4℃的含40g/L多聚甲醛、0.01%(体积分数)戊二醛、0.2%(体积分数)饱和苦味酸的磷酸缓冲液(0.1mol/LPB,pH7.4)。10min后取出脾,以不含戊二醛的上述固定液于4℃再固定12~16h。沿与脾长轴垂直方向将脾分为...
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