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ChargedResiduesoftheConservedDRYTripletoftheVasopressinV1aReceptorProvideMolecularDeterminantsforCellSurfaceDeliveryandInternalization
...tivationprocessandmediationofG-proteinsignaling.TheaimofthisstudywastodeterminewhetherspecificfeaturesatthislocusareimportantforthevasopressinV1areceptor(V1aR)byperformingsite-directedmutagenesis.IntransfectedHEK293Tcells,mutationofAsp(D148A)resultedinamisfoldedreceptorthatwasnonfunctional,localized...
医源资料库；在线期刊；分子药理学杂志；2006年第68卷第4期 -
HPLC测定吴茱萸及其制剂中吴茱萸碱和吴茱萸次碱含量
...包括：吴茱萸药材：流动相为乙腈-水(50∶50)，流速1.0ml.min-1，内标物为醋酸甲羟孕酮；华佗再造丸：流动相为乙腈-水(40∶60)，流速1.0ml.min-1，内标物为联苯；紫外检测波长均为225nm。结果：吴茱萸药材中吴茱萸碱和吴茱萸次碱的...
药品天地；专业药学；实验技术；色谱技术；色谱论文 -
BiochemicalandProteomicsApproachestoCharacterizeTopoisomeraseIICysteinesandDNAasTargetsResponsibleforCisplatin-InducedInhibitionofTopoisomer
...einegroupsinthetopoisomeraseIImonomerwereevaluatedbymassspectrometrytodeterminewhichcysteineswerefreeanddisulfide-bondedtoidentifypossiblesitesofcisplatinadduction.High-pressureliquidchromatography-matrix-assistedlaserdesorptionionizationmassspectrometryshowedthattopoisomeraseIIcontainedatleastfivef...
医源资料库；在线期刊；分子药理学杂志；2005年第67卷第3期 -
第三节 DNA重组实验中常用的技术
...养物倒入1.5ml离心管中，用台式离心机于4℃以12000g离心5min，将剩余的培养物贮存于4℃。 （3）吸尽培养液，使细菌沉淀尽可能干燥。 2．碱裂解法小量抽提质粒 （1）将细菌沉淀[上述步骤（3）所得]重悬于100μl预冷的...
医源资料库；医源图书馆；教材类；实用免疫细胞与核酸 -
ComparisonbetweentheHybridCaptureIITestandaPCR-BasedHumanPapillomavirusDetectionMethodforDiagnosisandPosttreatmentFollow-UpofCervicalIntr
...initialvolumeofapproximately1,000μl,werethawedandcentrifugedat3,000xgfor10minatroomtemperature.Thecellpelletwasresuspendedin1,000μlTris-HCl(10mM),pH7.4.Aliquotsofeachsamplewerefrozenat–20°C,thawedandboiledfor10min,centrifugedbrieflytoavoiddropletsatthetubetop,andfrozenat–20°Cuntilanalysiswas...
医源资料库；在线期刊；微生物临床杂志；2005年第43卷第7期 -
Lowry–ProteinDetermination
Lowry–ProteinDetermination(FromProteinProtocolsonCD-ROMHumanaPress,1998-Section1-2TheLowryMethodforProteinQuantitationJakobH.WaterborgandHarryR.Matthews)(Lowry,O.H.,Rosebrough,N.J.,Farr,A.L.,andRandall,R.J.(1951)ProteinmeasurementwiththeFolinphenolreagent.J.Biol.Chem.193,265-275)IntroductionTh...
药品天地；专业药学；实验技术；基本实验技术；生物分子定量 -
HumanImmunodeficiencyVirusType1inIllicit-DrugSolutionsUsedIntravenouslyRetainsInfectivity
...st-West(AFEW),MoscowPermRegionalAIDSCentre,Perm,RussianFederationWright-FlemingInstitute,ImperialCollegeLondon,UnitedKingdomABSTRACTThestabilityofthehumanimmunodeficiencyvirustype1(HIV-1)strainIIIBindrugsolutionswasstudied.ThedatademonstratethatHIV-1infectivitycanberetainedindrugsolutions(e.g.,heroi...
医源资料库；在线期刊；微生物临床杂志；2005年第43卷第4期 -
Anthocyaninsareabsorbedinglycatedformsinelderlywomen:apharmacokineticstudy
...werelongconsiderednonabsorbable.Objective:Theobjectiveofthisstudywastodeterminewhetheranthocyaninscanbeabsorbedasglycosidesandtoevaluatetheirpharmacokineticsinhumans.Design:Fourhealthyelderlywomenconsumed720mganthocyanins.Aseriesofbloodandurinesampleswerecollectedbeforeandafterconsumptionoftheanthoc...
医源资料库；在线期刊；美国临床营养学杂志；2001年73卷第5期 -
液相色谱/蒸发光散射测定转基因烟草提取液中海藻糖含量
...0.03%乙二胺的乙腈/水(体积比2.6:1)作为流动相，流速1.0mL/min；柱温25℃；进样量20mL；ELSD参数：漂移管温度85℃，氮气流速2.0L/min。2.结果与讨论2.1分离条件的选择在流动相里加入二胺或多胺，它们的氨基可以与硅胶表面的硅醇基产...
药品天地；专业药学；实验技术；色谱技术；色谱分析实例 -
3种麻醉方法在乳腺癌根治术的临床应用
...点选择T3-4或T4-5。穿刺成功后置管，给1.33%利多卡因5ml，5min后麻醉平面确切给入1.33%利多卡因与0.125%布比卡因合剂10～15ml，10～15min后复合静脉杜氟间隔追加安定2mg，氯胺酮20mg，40min左右硬膜外追加5ml局麻药合剂，术中血压下降，...
医源资料库；在线期刊；局解手术学杂志；2007年第16卷第1期