cIAP2isaubiquitinproteinligaseforBCL10andisdysregulatedinmucosa-associatedlymphoidtissuelymphomas

...b,andtheimmunoprecipitatedproteinsandcellextractswereanalyzedbyWesternblot(WB)withtheindicatedantibodies.Molecularweightstandards(inkDa)areshownontheleft.F,FLAG.(C)InteractionofcIAP2andBCL10invitro.Invitro–translated,35S-labeledcIAP2proteinswereincubatedwitheitherGSTproteinoraGSTfusionofBCL10immob...

InteractionoftheCa2+-sensingreceptorwiththeinwardlyrectifyingpotassiumchannelsKir4.1andKir4.2resultsininhibitionofchannelfunction

...ti-CaRantibodyorananti-Mycantibody(A-14).IP-AB,immunoprecipitatingantibody;WB-AB,Westernblottingantibody;No,IP-ABwasnotused.InteractionoftheCaRandKir4ishighlyselective.Kir4.2andKir4.1aretheonlytwoknownmembersoftheKir4familyandarebothexpressedinthedistalnephron,potentiallyinthesameregionastheCaR(19,2...

TargetedablationofIKK2improvesskeletalmusclestrength,maintainsmass,andpromotesregeneration

...komicewasperformedwithapolyclonalantibodyagainstNEMO,followedbyWesternblot(WB)analysiswithamonoclonalantibodyagainstIKK1orIKK2asindicated.MEF,mouseembryonicfibroblasts.(E)NucleartranslocationofNF-BwasdemonstratedbyEMSA.(F)EvaluationofIKKactivity.Totalprotein(300μg)fromnormalorchallenged(injuredorde...

Antiviralchemotherapyfacilitatescontrolofpoxvirusinfectionsthroughinhibitionofcellularsignaltransduction

...(+)indicatesSPGFadditiononly.TotalcelllysateswereanalyzedbyWesternblotting(WB)with4G10anti-phosphotyrosine(P-Y)mAb.ArrowsindicatepositionsofphosphorylatedsubstratesaffectedbyErbB-1inhibition.NumbersonleftsideofthepanelindicatethemolecularmarkerinkDa.(C)ErbB-1internalizationpreventedbyinhibitors.A431...

UltrasoundStimulatesCyclooxygenase-2ExpressionandIncreasesBoneFormationthroughIntegrin,FocalAdhesionKinase,Phosphatidylinositol3-Kinase,andAkt

...icforp85.ImmunoprecipitatedproteinswereseparatedbySDS-PAGEandimmunoblotted(WB)withanti-phosphotyrosine(PY)atvarioustimeintervalsafterUSstimulation.B,osteoblastswerepretreatedwithmAbagainst51orv3integrin(20µg/ml),rhodostomin(Rn,0.28µM)for30min,ortransfectedwithFAK(Y397F)mutantorpcDNA3(vec...

GATA-6regulatessemaphorin3Candisrequiredincardiacneuralcrestforcardiovascularmorphogenesis

...ld-typeGATA-6(373bp)andGATA-6followingexon4deletion(285bp).Westernblotting(WB)identifiesfull-length(45kDa)andtruncated(41kDa)GATA-6proteins.(E)ActivationoftheGATA-dependentDab2-LUCreporter.NIH3T3cellsweretransientlytransfectedwith100ngoftheDab2-LUCreporterandwith0.1–2.0μgofeitherpcDNA3–GATA-6or...

ConvergenceofHeatShockProtein0withUbiquitininFilamentous-SynucleinInclusionsof-Synucleinopathies

...brainsasdescribedabove.Figure4.RepresentativeimagesfromWesternblotanalyses(WB)ofHS,RIPA,andFAsamplesofcingulatecortexfromnormal(norm),PD(PD),DLBbrains(DLB),andponsofMSAbrain(MSA).EqualamountsofsamplewereanalyzedbySDS-polyacrylamidegelelectrophoresis.TheseresultsshowthattheFAfractionfromPD,DLB,andMSA...

RevascularizationofischemictissuesbyPDGF-CCviaeffectsonendothelialcellsandtheirprogenitors

...entwithPDGF-CCincreasedboththeformationandmaturation(viacoveragebySMCs)ofnewbloodvessels(Figure2,B,C,F,andG).Moreover,PDGF-CCenhancedskeletalmuscleregeneration(Figure2,EandH–L)and,asaresult,alsoreducedtheextentofischemicmusclenecrosis(Figure2,DandH–L),whichsuggeststhatmuscleregenerationandangiog...

Mastcellscanpromotethedevelopmentofmultiplefeaturesofchronicasthmainmice

...nistered24hoursaftertheeighthOVAchallengecomparedwithresponsesinPBS-treatedWBB6F1-Kit+/+miceorOVA-orPBS-treatedMC-deficientWBB6F1-KitW/W-vmice(Figure1A).However,WBB6F1-KitW/W-vmicethathadbeenselectivelyengraftedwithWBB6F1-Kit+/+bonemarrow–derivedculturedMCs(BMCMCs)(Kit+/+BMCMCsKitW/W-vmice)exhibit...

RenalexpressionoforganicaniontransporterOAT2inratsandmiceisregulatedbysexhormones

...HerewestudiedtheexpressionofOAT2mRNAbyRT-PCRanditsproteinbyWesternblotting(WB)andimmunocytochemistry(IC)inkidneysofadultintactandgonadectomizedMandF,sexhormone-treatedcastratedM,andprepubertalMandFrats,andtheproteininadultMandFmice.Inadultrats,theexpressionofOAT2mRNAwaspredominantintheouterstripe(OS...