EvaluationofNestedPCRinDiagnosisofTyphoidFever
...dforthediagnosisoftyphoidfever,itsresultsareoftenjeopardizedduetopriorinadequatedosesofantimicrobials(10).Thespecificgenesequenceofthebacterium,whichcanbeamplifiedanddetectedspecificallyandrapidlybynestedPCR(4,13),isanotherimportanttarget,havingthesensitivityofdetectingevenonebacteriuminagivensample...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第1期全颅常规外照射对大鼠血脑脊液屏障上Pgp和MRP1表达的影响及意义
...强,20、30、40Gy组与对照组比较,差异有显著性(F=8.18,q=4.496~6.670,P<0.05);10Gy组与20、30、40Gy组比较,差异有显著性(q=3.371~5.551,P<0.05)。MRP1在对照组有微弱表达,随着照射剂量的增加其表达有所增强,40Gy组与其他各组间比...
医源资料库;在线期刊;齐鲁医学杂志;2008年第23卷第2期UseoftheRestrictionEnzymeEcoRIforPulsed-FieldGelElectrophoreticAnalysisofHelicobacterpylori
...l,10mMEDTA,pH8.0),andresuspendedinPettIVsolution(1MNaCl,10mMEDTA,pH8.0).Anequalvolumeofmelted2.0%low-melting-pointagarose(LowMeltPreparativeGrade;Bio-RadLaboratories,Hercules,Calif.)wasaddedtothissuspension.Themixturewaspouredintoaninsertandchilledat4°Cfor20min.Themixturewasthentreatedat37°Cwith50...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第2期AnewcardiacMASTerswitchfortherenin-angiotensinsystem
...sinsystem(RAS)isahormonesysteminwhichthesubstrateproteinangiotensinogenissequentiallycleavedbypeptidases,reninandangiotensin-convertingenzyme(ACE),toformthebiologicallyactiveoctapeptideangiotensinII(Figure1).Asubstantialexcessofangiotensinogenispresentinserum,andACEisubiquitousintheendotheliumandpla...
医源资料库;在线期刊;临床研究杂志;2006年第116卷第4期OutbreaksofGastroenteritisThatOccurredduringSchoolExcursionsinKoreaWereAssociatedwithSeveralWaterborneStrainsofNorovirus
...ingroundwaterfromthehotels.Oftheseseveralstrainsofnorovirus,thenucleotidesequencesforonestrainwereidenticalforsamplesfromthestudents,foodhandlers,andgroundwater.TEXTNorovirusesarethemostcommoncauseofepidemicfood-andwaterborneviralgastroenteritis(5).Thepresenceofnorovirusesinwatersourcesisaparticular...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第9期RecruitmentofFoxp3+TregulatorycellsmediatingallografttolerancedependsontheCCR4chemokinereceptor
...,Foxp3,agenethatencodesaforkhead/wingedhelixtranscriptionfactorScurfin,isrequiredforTregcelldevelopmentandstudiessupportitsutilityasamarkerofTregcells(10,11).Ofthemultipleformsofcostimulationblockadethatcanpromotelong-termengraftmentinrodentmodels,onlytargetingofCD40–CD154interactions,typicallyinc...
医源资料库;在线期刊;实验医学杂志;2005年第201卷第7期第四节肺胀
...,目如脱等症状,认为本病与痰饮有关,开始应用越婢加半夏汤、小青龙加石膏汤等方药进行辨证论治。隋代《诸病源候论·咳逆短气候》记载肺胀的发病机理是由于“肺虚为微寒所伤则咳嗽,嗽则气还于肺间则肺胀,肺胀则...
医源资料库;医源图书馆;教材类;中医内科学;第二章 肺病证IdentificationofaSecondBlockerBindingSiteattheCytoplasmicMouthoftheCysticFibrosisTransmembraneConductanceRegulatorChlorideChannelPore
...macroscopicCFTRcurrentswererecordedaftermaximalPKAstimulation(20nM)andsubsequenttreatmentwithpyrophosphate(2mM)to“lock“channelsintheopenstate.Inallexperiments,theintracellular(bath)solutioncontained150mMNaCl,2mMMgCl2,and10mMN-tris(hydroxymethyl)methyl-2-aminoethanesulfonate(TES).Althoughafewexpe...
医源资料库;在线期刊;分子药理学杂志;2007年第69卷第5期RapidIdentificationofHumanAdenovirusTypes3and7fromRespiratorySpecimensviaMultiplexType-SpecificPCR
...diverseserotypesandprovedabletocorrectlyidentifyAd3andAd7.ThisassaywassubsequentlyappliedtothedetectionofAd3andAd7inrespiratoryspecimens.Amongthe127nasalaspiratesfromwhichanadenoviruswasgrown,thesensitivitywithwhichanyserotypecouldbedetectedwas91%(115/127).Twoofthe53nasalaspirateswhichdidnotgrowAdsy...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第11期HighFrequencyofGastricColonizationwithMultipleHelicobacterpyloriStrainsinVenezuelanSubjects
...idualhost.Usingenzyme-linkedimmunosorbentassayandlineprobeassay(LiPA)techniques,weanalyzedtheprevalenceofmixedH.pyloricolonizationin127subjectsfromVenezuela,acountryofhighH.pyloriprevalence,fromthreeregionsrepresentingdifferentpopulationgroups:theAndes(Merida),whereCaucasianmestizospredominate,amajo...
医源资料库;在线期刊;微生物临床杂志;2005年第43卷第6期