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丙型肝炎病毒包膜蛋白E1研究进展*
...中，前者获得了表达，后者不表达。表达产物经纯化，用WB检测，可以和用哺乳动物细胞表达的E1免疫后产生的抗体发生交叉反应。由此推断，原核表达的抗原和真核表达的抗原享有同样的抗原性［21］。近年来，核酸免疫技术的...
医源资料库；在线期刊；中华医学研究杂志；2007年第7卷第2期 -
NegativeRegulationofTCellReceptor–LipidRaftInteractionbyCytotoxicTLymphocyte–associatedAntigen4
...TLA-4wasimmunoprecipitated(IP)fromeachfractionanddetectedbyWesternblotting(WB)usingananti-CTLA-4antibody(1stpanel).Atthesametime,themembranewasprobedwithananti-phosphotyrosineantibody(anti-ptyr:2ndpanel).Theblotwasstrippedandreprobedbyananti-TCRantibody(3rdpanel).Thebottompaneldepictstheresultfromch...
医源资料库；在线期刊；实验医学杂志；2003年第197卷第1期 -
转基因悄然而胜
...网的上证博客就适时推出了“种子闹革新”的转基因题材攻略。丰乐种业、登海种业、隆平高科、亚盛集团等国内一干从事转基因生产和销售的上市公司受到热捧。 伴随着转基因重大专项的立项，争议不断的转基因水稻，由...
医药经济；生物技术；技术要闻 -
NegativeRegulationofTCellReceptor–LipidRaftInteractionbyCytotoxicTLymphocyte–associatedAntigen4
...TLA-4wasimmunoprecipitated(IP)fromeachfractionanddetectedbyWesternblotting(WB)usingananti-CTLA-4antibody(1stpanel).Atthesametime,themembranewasprobedwithananti-phosphotyrosineantibody(anti-ptyr:2ndpanel).Theblotwasstrippedandreprobedbyananti-TCRantibody(3rdpanel).Thebottompaneldepictstheresultfromch...
医源资料库；在线期刊；美国病理学杂志；2003年第162卷第1期 -
游泳引起的传染病流行及预防
...卫生学监测。美国从1971年开始建立了水源性疾病暴发（WBDOs）监测系统。该监测系统包括与饮用水和娱乐活动用水有关的疾病暴发的资料，主要目的是:描述WBDOs的流行病学特征;确定引起WB-DOs的病原以及暴发发生原因;培训公共卫...
医源资料库；在线期刊；中国热带医学杂志；2006年第6卷第8期 -
MolecularMechanismsUnderlyingtheProangiogenicEffectofFactorXIII
...ceofeitherFXIIIaorI-FXIIIweretestedfortyrosinephosphorylationbyWesternblot(WB)analysis.AsshowninFigure1A,FXIIIa,butnotI-FXIII,stimulatedtyrosinephosphorylationof2proteinswithMrof250and230kDa.AnotherphosphorylatedproteinwithMrof120kDawaspresentinuntreatedcellsandwasnotaffectedbyFXIIIaorI-FXIIItreatme...
医源资料库；在线期刊；动脉硬化血栓血管生物学杂志；2005年第25卷第3期 -
DetectionandLocalizationofPrPScintheSkeletalMuscleofPatientswithVariantIatrogenicandSporadicFormsofCreutzfeldt-JakobDisease
...ProfilesofCJDPatientswithMuscleShowntoBePositiveforPrPresbyWesternBlotting(WB)andPETBlottingPatientswithsCJDUsingthissameWesternblottingtechnique,6of26patientswithdefinitesCJDwereidentifiedasbeingpositiveforskeletalmusclePrPres(Figure2).Twoofthesepatients,S7andS10,werealsoshowntobepositivebyPETblott...
医源资料库；在线期刊；美国病理学杂志；2006年第168卷第3期 -
HSPA12BIsPredominantlyExpressedinEndothelialCellsandRequiredforAngiogenesis
...expressioninmultipletissuesandpreferentiallyinHUVEC,detectedbyWesternblots(WB).AtoC,Blotswerestrippedandre-probedwithanti-GAPDHmonoclonalantibodytoassessproteinloading.A,Validationoftheantibodies.WesternblotswithAb4110(left)andAb4112(right)astheprimaryantibodiesonwholecelllysates(10µg)wereperf...
医源资料库；在线期刊；动脉硬化血栓血管生物学杂志；2006年第26卷第9期 -
RenewalofMuralThrombusReleasesPlasmaMarkersandIsInvolvedinAorticAbdominalAneurysmEvolution
...omogeneousdistributionofTFthroughoutthethrombuslayerswasshownbyWesternblot(WB,n=6).TheprocoagulantmaterialreleasedbythethrombusconsistedinpartofTF,becauseablockingTFantibodyinducedasignificant(P<0.05)prolongationoftheclottingtimes,regardlessofthelayerstudied(Figure2A).Thispartialinhibitoryeffectofth...
医源资料库；在线期刊；美国病理学杂志；2006年第168卷第3期 -
TheMitochondrialK-ATPChannelOpenerDiazoxidePreventsIschemia-ReperfusionInjuryintheRabbitSpinalCord
...mountedwithappropriatemountingmedia.WesternBlotAnalysisWesternblotanalysis(WB)wasperformedaswepreviouslydescribed.26,31Tissuesampleswerehomogenizedinalysisbuffer(0.1mol/LNaCl,0.01mol/LTris-HCl,pH7.5,1mmol/LEDTA,and1µg/mlaprotinin),andthenthehomogenateswerecentrifugedat7000xgfor15minutesat4??C....
医源资料库；在线期刊；美国病理学杂志；2006年第168卷第5期