您是不是想找：
-
急性坏死性胰腺炎大鼠肺脏TNF-α变化及地塞米松干预作用
...1.7肺脏的TNF-α蛋白的表达蛋白质印迹法（Westernblot，WB）检测。具体方法：①取100g肺组织电动匀浆机匀浆，12000r/min离心5min后取上清。以牛血清白蛋白为标准蛋白，用Lowry法测定蛋白浓度，样品分装-70℃保存。②15%SDS-PAGE分离...
医源资料库；在线期刊；中国热带医学杂志；2007年第7卷第10期 -
CriticalRoleoftheCarboxylTerminusofProline-richTyrosineKinase(Pyk2)intheActivationofHumanNeutrophilsbyTumorNecrosisFactor
...oneasacontrol(C).TotalcelllysateswereseparatedbySDS-PAGEandwestern-blotted(WB)with(A)antiphosphotyrosineAbor(B)Absspecificforindividualphosphotyrosine(PY)residuesofPyk2.Onemembranewasstrippedandreprobedwithanti-Pyk2Ab,demonstratingequalloadingofthelanes. InhibitionofTNF-dependentAssociationofPyk2a...
医源资料库；在线期刊；美国病理学杂志；2003年第162卷第1期 -
CriticalRoleoftheCarboxylTerminusofProline-richTyrosineKinase(Pyk2)intheActivationofHumanNeutrophilsbyTumorNecrosisFactorSeparationofSig
...oneasacontrol(C).TotalcelllysateswereseparatedbySDS-PAGEandwestern-blotted(WB)with(A)antiphosphotyrosineAbor(B)Absspecificforindividualphosphotyrosine(PY)residuesofPyk2.Onemembranewasstrippedandreprobedwithanti-Pyk2Ab,demonstratingequalloadingofthelanes. InhibitionofTNF-dependentAssociationofPyk2a...
医源资料库；在线期刊；实验医学杂志；2003年第197卷第1期 -
ComparisonofTwoTargetGenesforDetectionandGenotypingofGiardialambliainHumanFecesbyPCRandPCR-RestrictionFragmentLengthPolymorphism
...ingGiardiaspeciesandgenotypes(GenBankaccessionnumber):G.lambliaassemblageA:WB(L02120),JH(U57897),Ad-1(AF069556),Ad-2(AF069557),isolate2907(AY228647),isolatefromwilddeer(AY302562);assemblageB:GS/M(L02116),BAH-12(AF069561),Ad-19(AF069560),isolate2924(AY228628),isolate2582(AY228629),isolate2506(AY22863...
医源资料库；在线期刊；微生物临床杂志；2005年第43卷第12期 -
InductionofMonocyticTissueFactorExpressionAfterRewarmingFromHypothermiaInVivoIsCounteractedbyHeatShockinc-Jun-DependentManner
...rich)atafinalconcentrationof10U/mL.FortheTFactivityassayandWesternblotting(WB),mononuclearcells(MNCs)wereisolatedfrombloodaliquotsbydensitycentrifugation.18Briefly,bloodaliquotswerepre-dilutedwithphysiologicalsaline,overlaidontoLymphoprep(Axis-Shield)andsubjectedtocentrifugationat415gfor15minutes.Af...
医源资料库；在线期刊；动脉硬化血栓血管生物学杂志；2006年第26卷第10期 -
第六节 诊断
...荧光检测法（IFA）；4.免疫印迹检测法（WesternBlot，简称WB法）；5.放射免疫沉淀法（RIP）。其中前三项常用于筛选试验，后二者用于确证试验。HIV感染后数周或数日内常不能检出抗体，95%的受染者在5个月内可测出抗体。但也有感...
医源资料库；医源图书馆；教材类；基因诊断与性传播疾病 -
MicroarrayAnalysisRevealsOverexpressionofCD163andHO-1inSymptomaticCarotidPlaques
...ngprobabilityvaluesaregivenbeloweachgraph.IHCindicatesimmunohistochemistry;WB,westernblotting.ResultsMicroarrayAnalysisinPatientsWithBilateralCarotidStenosesFortyprobes(33genes)showedmorethan1.5-foldchangeinexpressionincomparisonbetweenasymptomaticandsymptomaticplaques(summarizedinsupplementalTableI...
医源资料库；在线期刊；动脉硬化血栓血管生物学杂志；2007年第27卷第1期 -
重组人白细胞介素18在原核系统中高效表达的策略
...E.coli.MethodsTheyieldofcombinanthumanIL-18expressioninE.coliremainsquitelowbecausethesequenceofmaturehIL-18has37aararecondonsforE.coliinatotalof157aa.Toovercomethisproblem，genesynthesiswasperformedwithoptimizedcondonsfortheexpressionhostE.colithroughoverlapPCRusingsixsynthesisDNAfragmentsof95-101...
医源资料库；在线期刊；中华医药杂志；2007年第7卷第9期 -
RelativecontributionofOATandOCTtransporterstoorganicelectrolytetransportinrabbitproximaltubule
...entofradiolabeledsubstratetransport,CHOcellswereincubatedinWaymouthsbuffer[WB;(inmM)135NaCl,13HEPES-NaOH,pH7.4,28D-glucose,5KCl,1.2MgCl2,2.5CaCl2,and0.8MgSO4]towhichlabeledsubstrateplusappropriatetestagentswereadded.Incubationwasstoppedbyrinsingthecellsthreetimeswith2mlofice-coldWB.Thecellswerethens...
医源资料库；在线期刊；美国生理学杂志；2004年第287卷第11期 -
发育性髋脱位保守治疗方法和影响因素概述
...plasia.In：HerringJA.ed.Tachdjianspeditric.Orthopaedic3rdde.Philadephia：WB，SaundersCompany，2002，587-596. 8BlomHC，HeldaasO，ManoharanP，etal.UltrasoundscreeningforhipdysplasiainnewbornsandtreatmentwithFrejkapillow.TidsskrNorLaegeforen，2005，125(15)：1998-2001. 9McCulloughLM...
医源资料库；在线期刊；中华现代外科学杂志；2009年第6卷第10期