TheCannabinoidCB1ReceptorAntagonistRimonabant(SR141716)InhibitsHumanBreastCancerCellProliferationthroughaLipidRaft-MediatedMechanism
...x-wellplateswerewashedthreetimeswithInvitrogenprewarmedOpti-MEM.Toeachwell,100µlofOpti-MEMcontaining4µloflipofectinandoligonucleotides(1µMfinalconcentration)wasadded.Afterincubationfor4hat37°C,themediumwasreplacedwiththeappropriatecellgrowthmediumcontaining1µMofthesameoli...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第10期89锶治疗转移性骨肿瘤疼痛疗效分析
...2%。本组病例中2例前列腺癌、1例肺癌患者的骨转移瘤在89Sr治疗后3个月,SPECT显像检查显示,部分病灶放射性明显减低,部分病灶消失,疼痛完全缓解。进一步证实89Sr除有止痛效果外,还有一定的辐射治疗作用。2.2止痛起效和维...
医源资料库;在线期刊;中华现代临床医学杂志;2009年第7卷第6期SphingosineandItsAnalog,theImmunosuppressant2-Amino-2-(2-[4-octylphenyl]ethyl)-1,3-propanediol,InteractwiththeCB1CannabinoidReceptor
...ifugedat48,000g,andresuspendedinbufferB(50mMTris-HCl,3mMMgCl2,0.2mMEGTA,and100mMNaCl,pH7.4)andstoredinaliquotsat-80°C.Cerebellum.Micewerekilledbydecapitation,andcerebellaweredissectedoniceandplacedin20volumesofcoldbufferA.Cerebellawerehomogenizedandcentrifugedat48,000gat4°Cfor10min,andmembra...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第7期去甲肾上腺素对THP-1源性巨噬细胞SR-A1表达的影响
...以4×106个接种于25cm2培养瓶,每瓶含8ml培养基,再加入8μl100μmol/L佛波酯(Phorbol12-myristate13-acetate,PMA)使PMA终浓度为100nmol/L,37℃、5%CO2的培养箱中培养3天,显微镜下观察显示此时细胞呈贴壁状态,形状不规则并有伪足伸出,证...
医源资料库;在线期刊;中华中西医杂志;2006年第7卷第4期D2ReceptorsRegulateDopamineTransporterFunctionviaanExtracellularSignal-RegulatedKinases1and2-DependentandPhosphoinositide3Kinase-Independent
...inetheactivationofD2SRbytheDATsubstrates:DA(1pMto10µM),tyramine(1pMto100µM),andASP+(1pMto100µM).Cells(50,000cells/cm2)wereseededin96-wellplatesinDMEMmediasupplementedwith10%FBSandgrownat37°Cand5%CO2for24h.Tetracycline(0.1µM)wasaddedforanadditional16to20htoinduceD2SRexpres...
医源资料库;在线期刊;分子药理学杂志;2007年第69卷第5期AllostericModulationoftheCannabinoidCB1Receptor
...n),L-glutamine,Krebs‘salts,penicillin/streptomycin,Trisbuffer,andTritonX-100wereallobtainedfromSigma-Aldrich.[3H]CP55,940(128Ci/mmol)and[35S]GTPS(1250Ci/mmol)wereobtainedfromGEHealthcare(LittleChalfont,Buckinghamshire,UK).[3H]SR141716A(44Ci/mmol)wasobtainedfromtheNationalInstituteonDrugAbuse.Three...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第5期胃镜引导下光动力学微波化疗治疗上消化道恶性肿瘤的临床研究
...ang,ZhangHaitao,LiuManjia,etal. The454thHospitalofPLA,NanJing210002.【Abstract】ObjectiveToevaluatetheclinicaleffectandsaftyofphotodynamicscombinedwithchemotherapyandmicrowaveintreatingpatientswithgastrointestinalcancer.Methods693patientswithadvanceduppergastrointestiˉnalcancerweretr...
资料库;在线期刊;中华现代内科学杂志;2004年第1卷第3期Ligand-DirectedSignalingatthe3-AdrenoceptorProducedby3-(2-Ethylphenoxy)-1-[(1,S)-1,2,3,4-tetrahydronapth-1-ylamino]-2S-2-propanoloxalate(SR59230A)Re
...-12mediumcontaining10%(v/v)fetalbovineserum(FBS),glutamine(2mM),penicillin(100U/ml),andstreptomycin(100µg/ml).ClonalCHO-K1linestransfectedwiththe3-ARweregrownintheabovemediabutwiththeadditionofG418(400µg/ml).Cellsweremaintainedunder5%CO2at37°C,andcellswerepassagedevery3to4days.Radiol...
医源资料库;在线期刊;分子药理学杂志;2007年第69卷第11期ProlongedRecoveryRateofCB1ReceptorAdaptationafterCessationofLong-TermCannabinoidAdministration
....MembraneswereresuspendedinassaybufferA(50mMTris-HCl,3mMMgCl2,0.2mMEGTA,and100mMNaCl,pH7.4),andproteinwasdeterminedasdescribedpreviously(Selleyetal.,2004).Concentration-effectcurvesweregeneratedbyincubatingtheappropriateconcentrationofmembrane(5-10µg)inassaybufferAwith1g/lbovineserumalbumin(BS...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第9期EssentialRoleofaCa2+-Selective,Store-OperatedCurrent(ISOC)inEndothelialCellPermeability
...MeasurementsAllfluorescencemeasurementswereconductedusinganSLM-AMINCOModel8100high-resolutionspectrofluorometeranda10-mmpathlengthcellat23°C.InitialspectrabandwidthandwavelengthcalibrationswereperformedusinganNIST-traceablefluorescenceintensityset(WilmadGlass;cat.no.921-1).Excitationandemissionspec...
医源资料库;在线期刊;循环研究杂志;2005年第95卷第4期