ComparisonofTwoTargetGenesforDetectionandGenotypingofGiardialambliainHumanFecesbyPCRandPCR-RestrictionFragmentLengthPolymorphism

...ingGiardiaspeciesandgenotypes(GenBankaccessionnumber):G.lambliaassemblageA:WB(L02120),JH(U57897),Ad-1(AF069556),Ad-2(AF069557),isolate2907(AY228647),isolatefromwilddeer(AY302562);assemblageB:GS/M(L02116),BAH-12(AF069561),Ad-19(AF069560),isolate2924(AY228628),isolate2582(AY228629),isolate2506(AY22863...

InductionofMonocyticTissueFactorExpressionAfterRewarmingFromHypothermiaInVivoIsCounteractedbyHeatShockinc-Jun-DependentManner

...rich)atafinalconcentrationof10U/mL.FortheTFactivityassayandWesternblotting(WB),mononuclearcells(MNCs)wereisolatedfrombloodaliquotsbydensitycentrifugation.18Briefly,bloodaliquotswerepre-dilutedwithphysiologicalsaline,overlaidontoLymphoprep(Axis-Shield)andsubjectedtocentrifugationat415gfor15minutes.Af...

MicroarrayAnalysisRevealsOverexpressionofCD163andHO-1inSymptomaticCarotidPlaques

...ngprobabilityvaluesaregivenbeloweachgraph.IHCindicatesimmunohistochemistry;WB,westernblotting.ResultsMicroarrayAnalysisinPatientsWithBilateralCarotidStenosesFortyprobes(33genes)showedmorethan1.5-foldchangeinexpressionincomparisonbetweenasymptomaticandsymptomaticplaques(summarizedinsupplementalTableI...

RelativecontributionofOATandOCTtransporterstoorganicelectrolytetransportinrabbitproximaltubule

...entofradiolabeledsubstratetransport,CHOcellswereincubatedinWaymouthsbuffer[WB;(inmM)135NaCl,13HEPES-NaOH,pH7.4,28D-glucose,5KCl,1.2MgCl2,2.5CaCl2,and0.8MgSO4]towhichlabeledsubstrateplusappropriatetestagentswereadded.Incubationwasstoppedbyrinsingthecellsthreetimeswith2mlofice-coldWB.Thecellswerethens...

NegativeRegulationofTCellReceptor–LipidRaftInteractionbyCytotoxicTLymphocyte–associatedAntigen4

...TLA-4wasimmunoprecipitated(IP)fromeachfractionanddetectedbyWesternblotting(WB)usingananti-CTLA-4antibody(1stpanel).Atthesametime,themembranewasprobedwithananti-phosphotyrosineantibody(anti-ptyr:2ndpanel).Theblotwasstrippedandreprobedbyananti-TCRantibody(3rdpanel).Thebottompaneldepictstheresultfromch...

游泳引起的传染病流行及预防

...卫生学监测。美国从1971年开始建立了水源性疾病暴发（WBDOs）监测系统。该监测系统包括与饮用水和娱乐活动用水有关的疾病暴发的资料，主要目的是:描述WBDOs的流行病学特征;确定引起WB-DOs的病原以及暴发发生原因;培训公共卫...

MolecularMechanismsUnderlyingtheProangiogenicEffectofFactorXIII

...ceofeitherFXIIIaorI-FXIIIweretestedfortyrosinephosphorylationbyWesternblot(WB)analysis.AsshowninFigure1A,FXIIIa,butnotI-FXIII,stimulatedtyrosinephosphorylationof2proteinswithMrof250and230kDa.AnotherphosphorylatedproteinwithMrof120kDawaspresentinuntreatedcellsandwasnotaffectedbyFXIIIaorI-FXIIItreatme...

DetectionandLocalizationofPrPScintheSkeletalMuscleofPatientswithVariantIatrogenicandSporadicFormsofCreutzfeldt-JakobDisease

...ProfilesofCJDPatientswithMuscleShowntoBePositiveforPrPresbyWesternBlotting(WB)andPETBlottingPatientswithsCJDUsingthissameWesternblottingtechnique,6of26patientswithdefinitesCJDwereidentifiedasbeingpositiveforskeletalmusclePrPres(Figure2).Twoofthesepatients,S7andS10,werealsoshowntobepositivebyPETblott...

HSPA12BIsPredominantlyExpressedinEndothelialCellsandRequiredforAngiogenesis

...expressioninmultipletissuesandpreferentiallyinHUVEC,detectedbyWesternblots(WB).AtoC,Blotswerestrippedandre-probedwithanti-GAPDHmonoclonalantibodytoassessproteinloading.A,Validationoftheantibodies.WesternblotswithAb4110(left)andAb4112(right)astheprimaryantibodiesonwholecelllysates(10µg)wereperf...

RenewalofMuralThrombusReleasesPlasmaMarkersandIsInvolvedinAorticAbdominalAneurysmEvolution

...omogeneousdistributionofTFthroughoutthethrombuslayerswasshownbyWesternblot(WB,n=6).TheprocoagulantmaterialreleasedbythethrombusconsistedinpartofTF,becauseablockingTFantibodyinducedasignificant(P<0.05)prolongationoftheclottingtimes,regardlessofthelayerstudied(Figure2A).Thispartialinhibitoryeffectofth...