Preventionofobesityinmicebyantisenseoligonucleotideinhibitorsofstearoyl-CoAdesaturase–1
...nculturedprimarymousehepatocytes.PrimaryhepatocytesisolatedandculturedfromC57/B6miceweretransfectedwithvaryingconcentrations(50–800nM)ofSCD1-specificASOs(ASO1andASO2)andascrambledcontrolASO(ASOctrl).Afterthemicerecoveredovernight,RNAandproteinswereextractedfromthetransfectedcells.TheRNAwasusedtopr...
医源资料库;在线期刊;临床研究杂志;2005年第115卷第4期小鼠B7-1基因转染的黑色素瘤细胞免疫原性探讨
...验研究。 1 材料与方法 1.1 实验动物和细胞 C57BL/6小鼠,6~8周龄,雌性,购自上海西普尔-必凯实验动物有限公司。小鼠黑色素瘤细胞系B16(F0)(B16-wt,源于C57BL/6小鼠)购自武汉大学;B16-mB7.1和B16-neo系本室建立...
合作平台;医学论文;临床医学与专科论文;检验医学DeletionofRD1fromMycobacteriumtuberculosisMimicsBacilleCalmette-GuérinAttenuation
...ophagelikecelllineTHP-1,humanperipheralbloodmonocytederivedmacrophages,andC57BL/6mice.Ineachofthesesystems,theH37Rv:RD1strainwasstrikinglylessvirulentthanMTBandwasverysimilartoBCGcontrols.Therefore,itwasconcludedthatgeneswithinorcontrolledbyRD1areessentialforMTBvirulenceandthatlossofRD1wasimportanti...
医源资料库;在线期刊;传染病学杂志;2003年第187卷第1期同种移植激活的宿主树突状细胞能介导免疫耐受*
...受900rad致死量的伽玛射线照射,射线源为北京大学医学部钴-60照射室,然后于照射后的6h内,每只BALB/c受者,通过尾静脉,或者输入1.0×106骨髓细胞,或者输入1.0×106骨髓细胞和5.0×106激活的宿主DCs细胞的混合细胞。1.6移植和耐...
医源资料库;在线期刊;中华医学研究杂志;2006年第6卷第6期Nonneutralizingantibodiesbindingtothesurfaceglycoproteinoflymphocyticchoriomeningitisvirusreduceearlyvirusspread
...darerecognizedbyidiotypicantibodiesspecificformAbKL25(32).BindingofKL25toMC57Gcellsinfectedwithdifferentvirusvariantswasdeterminedbyflowcytometry.WecorrectedfordifferingGPsurfaceexpressionlevelsbynormalizingflowcytometricmeasurementswithparallelstainingsusingmAbWEN1.3,whichbindsequallywelltotheGPofe...
医源资料库;在线期刊;实验医学杂志;2006年第203卷第8期DexamethasoneInducesConnectiveTissueGrowthFactorExpressioninRenalTubularEpithelialCellsinaMouseStrain-SpecificManner
...n,theglucocorticoiddexamethasone(DEX)increasedCTGFmRNAlevelsinthekidneysofC57B6butnotSJLmiceandproducedintermediateCTGFmRNAlevelsinthekidneysofF1(C57B6xSJL)mice,midwaybetweenthelevelsfoundforparentalstrains.DEXalsoincreasedCTGFmRNAlevelsinculturedtubularepithelialcellsderivedfromC57B6(mProx24)butnot...
医源资料库;在线期刊;美国病理学杂志;2006年第168卷第3期HematopoieticStemCellsProvideRepairFunctionsafterLaser-InducedBruch??sMembraneRuptureModelofChoroidalNeovascularization
...s,retinalpigmentepithelia(RPE),macrophages,andpericytes.LethallyirradiatedC57BL6/JmicewerereconstitutedwithHSCsfrommicehomozygousforgreenfluorescentprotein(GFP)andthensubjectedtolaser-inducedruptureofBruch??smembrane.Afterimmunohistochemicalexaminationofoculartissue,GFP+astrocyteswereobservedconcent...
医源资料库;在线期刊;美国病理学杂志;2006年第168卷第3期2.幽门螺杆菌的基础实验研究--大鼠实验性幽门螺杆菌感染
...购自中国医学科学院实验动物研究所。新西兰种家兔,C57和BALB/小鼠,由本所动物室供给。 1.3感染方法Wistar大鼠的实验分2次进行。第1次用出生5个月(体重175g~254g)的成年鼠,第2次用出生两个月(体重152g~174g)的幼龄鼠...
医源资料库;医源图书馆;教材类;中国幽门螺杆菌研究复方感冒冲剂体外抗流感病毒作用
...的形态学观察[J].湖北工学院学报,2000,15(3):56~57. [2]杨建林,樊晓晖.流感病毒引起细胞凋亡因素的研究进展[J].中国热带医学,2006,6(9):1676~1678. [3]潘英.复方感冒颗粒剂的制备及临床观察[J].医学文...
医源资料库;在线期刊;中国热带医学杂志;2007年第7卷第6期EffectsofangiotensinIIontheCO2dependenceofHCO3
...iatedcarbonicanhydrasetypeIVinkidneyepithelialcells.ProcNatlAcadSciUSA87:74577461,1990.BurgM,GranthamJ,AbramowM,andOrloffJ.Preparationandstudyoffragmentsofsinglerabbitnephrons.AmJPhysiol210:12931298,1966.BurgMandGreenN.Bicarbonatetransportbyisolatedperfusedrabbitproximalconvolutedtubules.AmJPhysiolR...
医源资料库;在线期刊;美国生理学杂志;2006年第289卷第2期