Mechanismsofprogressionandregressionofrenallesionsofchronicnephropathiesanddiabetes
...verywere3Dreconstructionstudiesoftheglomerularcapillarytuft,whichallowedthequantificationofsclerosisvolumereductionandcapillaryregenerationupontreatment.Regenerationofcapillarysegmentsmightresultfromthecontributionofresidentcells,butprogenitorcellsofrenalorextrarenaloriginmayalsohavearole.Thisreview...
医源资料库;在线期刊;临床研究杂志;2006年第116卷第2期Small-moleculecorrectorsofdefectiveF508-CFTRcellularprocessingidentifiedbyhigh-throughputscreening
...tivecompoundsyieldedseveralclassesofF508-CFTRcorrectors(aminoarylthiazoles,quinazolinylaminopyrimidinones,andbisaminomethylbithiazoles)withmicromolarpotencythatproducedgreaterapicalmembranechloridecurrentthandidlow-temperaturerescue.Correctionwasseenwithin3–6hoursandpersistedformorethan12hoursafte...
医源资料库;在线期刊;临床研究杂志;2005年第115卷第9期Invivodeterminationofdiffusivetransportparametersinasuperfusedtissue
...ozen,dried,slicedwithacryomicrotome,andexaminedwithfluorescentmicroscopyandquantitativeautoradiography.Themicrovesseldensity(x103/cm2:NE,50±10;C,180±7.0;NP,225±15)resultedinmarkeddifferences(P0.05)inwaterflux(µl·min-1·cm-2:NE,0.1±0.1;C,1.6±0.4;N...
医源资料库;在线期刊;美国生理学杂志;2006年第289卷第11期FunctionalinfluenceofN-glycosylationinOCT2-mediatedtetraethylammoniumtransport
...nceglycosylationhasonOCT2function,remainsunknown.Toaddresstheseissues,theacquisitionofN-glycosylationwasdisruptedbymutatingtheaminoacidasparagine(N)toglutamine(Q)atthesesitesintherabbitorthologofOCT2,whichwasexpressedinChinesehamsterovarycells.DisruptionofN-glycosylationfollowedbyWesternblottingindi...
医源资料库;在线期刊;美国生理学杂志;2006年第289卷第5期Noaudiblewheezing:nuggetsandconundrumsfrommouseasthmamodels
...Tlymphocytes(6).EffectorTlymphocytesarenecessaryandsufficienttoprovidetherequisiteTh2cytokinesthatinducebothhistologicchangesandinducedAHR.Theuseofknock-outmiceand/orblockadewithspecificantibodiesinwild-typemicerevealedthatIL-4signalingthroughtheIL-4receptorsubunit(IL-4R),andsubsequentSTAT6-dependen...
医源资料库;在线期刊;实验医学杂志;2005年第201卷第12期TheH+-LinkedMonocarboxylateTransporter(MCT1/SLC16A1):APotentialTherapeuticTargetforHigh-RiskNeuroblastoma
...lgorithmsasdescribedpreviously(Maris,2005).Biopsysamplesweresnap-frozeninliquidnitrogen,andadiagnosisofneuroblastomawasconfirmedbycentralpathologicreview.MYCNgenecopynumberstatuswasdeterminedbyfluorescencehybridizationinsitu;DNAindexwasdeterminedbyflowcytometryaftercelldissociation(Looketal.,1991;Ma...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第12期7-1线粒体
...主要有:黄素蛋白、细胞色素、铜原子、铁硫蛋白、辅酶Q等。 1.NAD即烟酰胺嘌呤二核苷酸(nicotinamideadeninedinucleotide,图7-4),是体内很多脱氢酶的辅酶,连接三羧酸循环和呼吸链,其功能是将代谢过程中脱下来的氢交给黄...
医源资料库;医源图书馆;教材类;细胞生物学AtrialnatriureticpeptidepromotescardiomyocytesurvivalbycGMP-dependentnuclearaccumulationofzyxinandAkt
...ialnatriureticpeptide(ANP)stimulationleadingtoelevationofcGMPlevelsandsubsequentnuclearaccumulationofAktkinaseassociatedwithzyxin,acytoskeletalLIM-domainprotein.NucleartargetingofzyxininducesresistancetocelldeathcoincidentwithnuclearaccumulationofactivatedAkt.NucleartranslocationofzyxintriggeredbycG...
医源资料库;在线期刊;临床研究杂志;2005年第115卷第10期MSH2–MSH6stimulatesDNApolymerase,suggestingaroleforA:Tmutationsinantibodygenes
...smthatgeneratesmutationsatA:Tbasepairs,however,remainsunclear.ItappearstorequiretheMSH2–MSH6mismatchrepairheterodimerandDNApolymerase(pol),asmutationsofA:Taredecreasedinmiceandhumanslackingtheseproteins.Here,wedemonstratethattheseproteinsinteractphysicallyandfunctionally.First,weshowthatMSH2–MSH...
医源资料库;在线期刊;实验医学杂志;2005年第201卷第4期Aninterstitialdeletion-insertioninvolvingchromosomes2p25.3andXq27.1,nearSOX3,causesX-linkedrecessivehypoparathyroidism
...hypoparathyroidism,duetoparathyroidagenesis,hasbeenmappedtoa906-kbregiononXq27thatcontains3genes(ATP11C,U7snRNA,andSOX3),andanalyseshavenotrevealedmutations.Wethereforecharacterizedthisregionbycombinedanalysisofsinglenucleotidepolymorphismsandsequence-taggedsites.Thisidentifieda23-to25-kbdeletion,wh...
医源资料库;在线期刊;临床研究杂志;2005年第115卷第10期