Contrastingfrequenciesofantitumorandanti-vaccineTcellsinmetastasesofamelanomapatientvaccinatedwithaMAGEtumorantigen
...withprimerscorrespondingtotheVandJ-Cregionsoftheevaluatedclonotype.Finally,0.001%ofthisproductwasusedforathirdamplification(35cycles),highlyspecificforapreciseclonotype,usuallywithaVprimerdifferentfromthoseusedinthepreviousamplificationsandaprimerstraddlingtheCDR3/Jjunctionwithoutcoveringitcompletel...
医源资料库;在线期刊;实验医学杂志;2005年第201卷第2期TranslationalregulationofvascularendothelialgrowthfactorexpressioninrenalepithelialcellsbyangiotensinII
...drogenperoxideinmethanoltoblocknonspecificperoxidaseactivityand0.01%avidin,0.001%biotintoblockendogenousbiotinactivity.Primaryandsecondantibodieswereincubatedfor30minwiththreewashesofPBScontaining0.1%BSAbetweensteps.ControlsconsistedofnonimmunerabbitIgGorPBS/BSAinplaceofprimaryantibodyfollowedbydete...
医源资料库;在线期刊;美国生理学杂志;2005年第288卷第3期ApolipoproteinsC-IIIandA-VasPredictorsofVery-Low-DensityLipoproteinTriglycerideandApolipoproteinB-100Kinetics
...re.Thedriedsamplewasthenreconstitutedin50µLofsamplebuffer(8mol/Lurea;0.001%wt/volbromophenolblue).ApoC-IIIfromVLDLwasisolatedbypreparativeisoelectricfocusinggelelectrophoresis(8mol/Lurea;7.5%acrylamide;1.5%ampholytes[pH4to6];16hours,200V,4°C).14Thegelswereelectroblottedontopolyvinylidenefl...
医源资料库;在线期刊;动脉硬化血栓血管生物学杂志;2006年第26卷第3期InteractionandInhibitoryCross-TalkbetweenEndothelinandErbBReceptorsintheAdultHeart
...oprecipitateswereelutedbysamplebuffer(51mMTris-HCl,4%SDS,4Murea,5%glycerol,0.001%bromphenolblue,and1%-mercaptoethanol)byboilingfor3min.CoimmunoprecipitatedproteinswereseparatedbySDS-PAGE.TocontrolfornonspecificbindingtoIgGbeadsandothersourcesofspuriousmassspectrometrypeaks,thesameimmunoprecipitation...
医源资料库;在线期刊;分子药理学杂志;2007年第69卷第6期RoleoftheforkheadproteinFoxO1in?cellcompensationtoinsulinresistance
...measuredratesof?cellproliferationandapoptosis.Onlyoccasionalapoptoticcells(0.001%of?cells)werepresentinallmiceanalyzed,andwedidnotdetectdifferencesamongthevariousgenotypes.Proliferating?cells,asmeasuredbyKi67incorporation,wererareinWT,307,L1,andL1/307mice(0.01%of?cells)butwerecompletelyabsentin305an...
医源资料库;在线期刊;临床研究杂志;2006年第116卷第3期AutophagicCardiomyocyteDeathinCardiomyopathicHamstersandItsPreventionbyGranulocyteColony-StimulatingFactor
...ssimilarbetweentheuntreatedandG-CSF-treatedUM-X7.1hamsters(untreated,0.06??0.001%versusG-CSF,0.04??0.008%,P=n.s.)(Figure4A).ActivationofDownstreamSignalsofG-CSFThebindingofG-CSFtoitsreceptorevokessignaltransductionthroughactivationofJanuskinase(Jak)/Stat;Aktkinase,whichhasbeenidentifiedasadownstream...
医源资料库;在线期刊;美国病理学杂志;2006年第168卷第2期生物防腐剂及其在食品防腐中的应用
...酵母及霉菌生长的作用。植物精油在植物中的含量一般为0.001%~10%,少数可达到20%。植物精油对于革兰氏阳性菌的抑制作用要强于对革兰氏阴性菌的抑制作用,也有许多植物的挥发性物质(如牛至、丁香、桂皮)对于这两类细菌...
医药经济;生物技术;技术要闻Interleukin-7isnecessarytomaintaintheBcellpotentialincommonlymphoidprogenitors
...thenumberofpro–Bcellsintheseanimalsisevenmoremarked:0.8%ofcontrolat4wkand0.001%at7wkaspublishedpreviously(25).Weshowedthatalreadyat4wkofage,IL-7–/–CLPshavereducedamountsofEbf.TheamountofEbfandPax5becomedrasticallyreducedinadultCLPs,whereasGata-3expressionisunaffected.Thus,IL-7signalingisnecess...
医源资料库;在线期刊;实验医学杂志;2005年第201卷第6期Effectsofexpressionofp53andGadd45onosmotictoleranceofrenalinnermedullarycells
...al)wasmadeupof2.5mlofdeionizedwater,4gofspeciallow-salt(NaCl)syntheticfood[0.001%Na(wt/wt);ResearchDiets(NewBrunswick,NJ)],15mgNaCl,and65mgofagar.Drinkingwaterwasprovidedadlibitumduringtheinitialperiodofthestudy.After3daysofadaptationtothecagesanddiet,urinewascollectedundermineraloilincollectionvial...
医源资料库;在线期刊;美国生理学杂志;2006年第289卷第8期TargetGenesofNeuron-RestrictiveSilencerFactorAreAbnormallyUp-RegulatedinHumanMyotilinopathy
...lyhomogenizedinafinalvolumeof1:20(w/v)inlysisbuffer(75mmol/LTris-HCl,pH6.8,0.001%bromphenolblue,15%sodiumdodecylsulfate,20%glycerol,5%ß-mercaptoethanol,1mmol/Lphenylmethylsulfonylfluoride,and1µg/mlaprotinin,leupeptin,andpepstatin).Sampleswereboiledat95??Cfor3minutesandcentrifugedat9500rp...
医源资料库;在线期刊;美国病理学杂志;2007年第169卷第10期